Prevention of retrovirus-induced aberrant cytokine secretion, excessive lipid peroxidation, and tissue vitamin E deficiency by T cell receptor peptide treatments in C57BL/6 mice

Bailin Liang, Zhen Zhang, Mohsen Araghiniknam, Cleamond Eskelson, Ronald R. Watson

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

To test whether T cell receptor (TCR) peptide treatment can prevent immune dysfunction, excessive lipid peroxidation, and malnutrition caused by retrovirus infection, female C57BL/6 mice were infected with LP-BM5 retrovirus. Infection with retrovirus inhibited lymphocyte proliferation, cytokine release T helper 1 cells, stimulated cytokine secretion by T helper 2 cells, induced abnormal hepatic and cardiac lipid profiles, and produced excessive tissue lipid peroxidation with hepatic and cardiac vitamin E deficiency. Two weeks after infection, TCR peptides Vβ5.2, Vβ8.1, Vβ8.1 + Vβ5.2, Vβ8.1(N), and Vβ8.1 were injected to the mice at dose of 200 μg/mouse. Vβ8.1 and Vβ5.2 treatments largely maintained lymphocyte proliferation and IL-2 and IFN-γ release, and prevented excessive IL-6, IL- 10, and TNF-α secretion. Concomitantly, these treatments normalized hepatic and cardiac lipid profiles, reduced tissue lipid peroxidation, and thereby significantly maintained vitamin E in the liver and heart. Vβ8.1 segments treatment did not prevent the immune dysfunction, abnormal lipid profile and lipid peroxidation, and vitamin E deficiency caused by the retrovirus infection. In conclusion, injection of intact TCR peptides during murine retrovirus infection largely prevented immune dysfunction by blocking the excessive stimulation of a T cell subset caused by retroviral superantigens. It also ameliorated malnutrition status by normalizing lipid profile, lipid peroxidation, and vitamin E deficiency. T cell immune dysfunction and its prevention by TCR peptide treatment is important in the therapy of vitamin E deficiency induced by retrovirus infection.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Volume214
Issue number1
DOIs
StatePublished - Jan 1997

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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