Preventory by L(-) ascorbic acid of isoproterenol-induced cardiotoxicity in primary cultures of rat myocytes

Kenneth Ramos, Daniel Acosta

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Primary cultures of rat myocytes were exposed to various doses of L-isoproterenol (ISO) for 4, 12 and 24 h. L-ascorbic acid (AA) was added to some cultures immediatedly after exposure to ISO to determine if antioxidants reduce the toxicity caused by ISO. Leakage of lactate dehydrogenase (LDH) and cell viability were used as indices of cell injury. A significant increase in LDH release was found 24 h after exposure to 1 × 10-4 M ISO alone. Higher doses (5 × 10-4 and 1 × 10-3 M) caused significant enzyme release 4, 12 and 24 h after exposure. The viability of cultures exposed to toxic doses of ISO for 4 h was not affected. A dose-dependent decrease in cell viability was observed 12 h and 24 h after exposure to ISO. L-ascorbic acid (5 × 10-3 and 1.5 × 10-2 M) significantly reduced the LDH release caused by ISO. The enzyme release from cultures exposed to 5 × 10-4 and 5 × 10-3 M AA alone was similar to that of control cultures. Howecer, 1.5 × 10-2 and 3 × 10-2 M AA caused marked LDH release. The viability of cultures subjected to ISo in the presence of 5 × 10-3 M AA was similar to that of controls. Our results show that AA protects against the LDH release and decreased cellular viability cuased by toxic doses of ISO.

Original languageEnglish (US)
Pages (from-to)81-90
Number of pages10
JournalToxicology
Volume26
Issue number1
DOIs
StatePublished - Jan 1983
Externally publishedYes

Keywords

  • Ascorbic acid
  • Cardiotoxicity
  • LDH release
  • Myocyte culturesl Isoproterenol

ASJC Scopus subject areas

  • Toxicology

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