Primary structure and developmental expression of a large cytoptasmic domain form of Xenopus laevis neural cell adhesion molecule (NCAM)

Paul A Krieg, Don S. Sakaguchi, Christopher R. Kintner

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The neural cell adhesion molecule, (NCAM), is involved in cell-cell interactions during development of the vertebrate nervous system. NCAM exists in multiple protein forms and these are selectively expressed in different cells and at different times during development. Here we report the complete amino acid sequence of the large cytoplasmic form of Xenopus laevis NCAM, derived from a full-length cDNA clone. Using specific probes the expression of different NCAM transcripts during, Xenopus embryogenesis has been examined. We find that transcripts encoding the large cytoplasmic domain form of NCAM exist in maternal RNA and that these are the only significant NCAM transcripts present until late gastrula when transcripts encoding the small cytoplasmic domain form of NCAM are first detected. No RNA encoding the small surface domain form of NCAM is detected during early development. These results indicate that the expression of NCAM sequences during early development of Xenopus differs from that described in other species.

Original languageEnglish (US)
Pages (from-to)10321-10335
Number of pages15
JournalNucleic Acids Research
Volume17
Issue number24
DOIs
StatePublished - Dec 25 1989
Externally publishedYes

Fingerprint

Adhesion Molecules
Cell Adhesion
Neural Cell Adhesion Molecules
Cell adhesion
Xenopus laevis
Molecules
Encoding
Xenopus
RNA
Cell
Embryogenesis
Form
Adhesion
Gastrula
Neurology
Amino Acid Sequence
CDNA
Clone
Cell Communication
Nervous System

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Primary structure and developmental expression of a large cytoptasmic domain form of Xenopus laevis neural cell adhesion molecule (NCAM). / Krieg, Paul A; Sakaguchi, Don S.; Kintner, Christopher R.

In: Nucleic Acids Research, Vol. 17, No. 24, 25.12.1989, p. 10321-10335.

Research output: Contribution to journalArticle

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