Primary structure of the DNA terminal protein of bacteriophage PRD1

Jui-Cheng Hsieh, Guhung Jung, Mark C. Leavitt, Junetsu Ito

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.

Original languageEnglish (US)
Pages (from-to)8999-9009
Number of pages11
JournalNucleic Acids Research
Volume15
Issue number21
DOIs
StatePublished - Nov 11 1987

Fingerprint

Bacteriophages
DNA
Proteins
Protein
Genes
Binding sites
Ribosomes
Bacteriophage PRD1
Amino acids
Binding Sites
Gene
Amino Acids
DNA Primers
Initiator Codon
Amino Acid Sequence
Nucleotides
Lipids
Promoter
Base Pairing
Tyrosine

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Primary structure of the DNA terminal protein of bacteriophage PRD1. / Hsieh, Jui-Cheng; Jung, Guhung; Leavitt, Mark C.; Ito, Junetsu.

In: Nucleic Acids Research, Vol. 15, No. 21, 11.11.1987, p. 8999-9009.

Research output: Contribution to journalArticle

Hsieh, Jui-Cheng ; Jung, Guhung ; Leavitt, Mark C. ; Ito, Junetsu. / Primary structure of the DNA terminal protein of bacteriophage PRD1. In: Nucleic Acids Research. 1987 ; Vol. 15, No. 21. pp. 8999-9009.
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