Abstract
The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8999-9009 |
| Number of pages | 11 |
| Journal | Nucleic Acids Research |
| Volume | 15 |
| Issue number | 21 |
| DOIs | |
| State | Published - Nov 11 1987 |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics
Cite this
Primary structure of the DNA terminal protein of bacteriophage PRD1. / Hsieh, Jui-Cheng; Jung, Guhung; Leavitt, Mark C.; Ito, Junetsu.
In: Nucleic Acids Research, Vol. 15, No. 21, 11.11.1987, p. 8999-9009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Primary structure of the DNA terminal protein of bacteriophage PRD1
AU - Hsieh, Jui-Cheng
AU - Jung, Guhung
AU - Leavitt, Mark C.
AU - Ito, Junetsu
PY - 1987/11/11
Y1 - 1987/11/11
N2 - The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.
AB - The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.
UR - http://www.scopus.com/inward/record.url?scp=0023651463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023651463&partnerID=8YFLogxK
U2 - 10.1093/nar/15.21.8999
DO - 10.1093/nar/15.21.8999
M3 - Article
C2 - 3684578
AN - SCOPUS:0023651463
VL - 15
SP - 8999
EP - 9009
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 21
ER -