TY - JOUR
T1 - Primary structure of the DNA terminal protein of bacteriophage PRD1
AU - Hsieh, Jui Cheng
AU - Jung, Guhung
AU - Leavitt, Mark C.
AU - Ito, Junetsu
N1 - Funding Information:
ACKNOWLEDCTMHJTS We are grateful to Dr. Leonard Mindich for supplying phage PRD1 and strains. We are also grateful to Dr. H. Bernstein and Connie Innis for critical reading of this manuscript. This research was supported by National Institute of Health Grant OE8013.
PY - 1987/11/11
Y1 - 1987/11/11
N2 - The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.
AB - The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.
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U2 - 10.1093/nar/15.21.8999
DO - 10.1093/nar/15.21.8999
M3 - Article
C2 - 3684578
AN - SCOPUS:0023651463
VL - 15
SP - 8999
EP - 9009
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 21
ER -