Primary structure of the DNA terminal protein of bacteriophage PRD1

Jui Cheng Hsieh; Guhung Jung; Mark C. Leavitt; Junetsu Ito

doi:10.1093/nar/15.21.8999

Primary structure of the DNA terminal protein of bacteriophage PRD1

Jui Cheng Hsieh, Guhung Jung, Mark C. Leavitt, Junetsu Ito

Basic Medical Sciences

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.

Original language	English (US)
Pages (from-to)	8999-9009
Number of pages	11
Journal	Nucleic acids research
Volume	15
Issue number	21
DOIs	https://doi.org/10.1093/nar/15.21.8999
State	Published - Nov 11 1987

ASJC Scopus subject areas

Genetics

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@article{67d880269f854d6298baae25c61a8437,

title = "Primary structure of the DNA terminal protein of bacteriophage PRD1",

abstract = "The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , {\o}29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and {"}-10{"} region.",

author = "Hsieh, {Jui Cheng} and Guhung Jung and Leavitt, {Mark C.} and Junetsu Ito",

note = "Funding Information: ACKNOWLEDCTMHJTS We are grateful to Dr. Leonard Mindich for supplying phage PRD1 and strains. We are also grateful to Dr. H. Bernstein and Connie Innis for critical reading of this manuscript. This research was supported by National Institute of Health Grant OE8013.",

year = "1987",

month = nov,

day = "11",

doi = "10.1093/nar/15.21.8999",

language = "English (US)",

volume = "15",

pages = "8999--9009",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

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T1 - Primary structure of the DNA terminal protein of bacteriophage PRD1

AU - Hsieh, Jui Cheng

AU - Jung, Guhung

AU - Leavitt, Mark C.

AU - Ito, Junetsu

N1 - Funding Information: ACKNOWLEDCTMHJTS We are grateful to Dr. Leonard Mindich for supplying phage PRD1 and strains. We are also grateful to Dr. H. Bernstein and Connie Innis for critical reading of this manuscript. This research was supported by National Institute of Health Grant OE8013.

PY - 1987/11/11

Y1 - 1987/11/11

N2 - The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.

AB - The genome of a lipid-containing phage, PRD1, is replicated by a proteinpriming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial hamlogy with that of any known texminal protein. However, hydropathy profiles of the FRDl , ø29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A prticular tyrosine residue is predicted to be covalently linked to the 5′ end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.

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