Primary structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum

Woo Ho-Hyung, Lindy A. Brigham, Martha C. Hawes

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The complementary DNA (PsUBC4) representing an mRNA encoding an ubiquitin-conjugating enzyme (UBC) of Pisum sativum has been cloned. The coding region is 444 nucleotides (nt) in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.95. The deduced aa sequence showed 97% identity with Arabidopsis thaliana AtUBC8-12 families and tomato ERT17, and 80% identity with yeast ScUBC4 and ScUBC5 and Drosophila melanogaster DmUBC4. The active site cysteine (Cys85) found in UBCs so far described is also conserved in the P. sativum sequence.

Original languageEnglish (US)
Pages (from-to)369-370
Number of pages2
JournalGene
Volume148
Issue number2
DOIs
StatePublished - Oct 21 1994

Keywords

  • Amino-acid sequence conservation
  • pea
  • plants

ASJC Scopus subject areas

  • Genetics

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