Priming for T-cell-mediated rejection of established tumors by cutaneous DNA immunization

Howard M. Ross, Lawrence W. Weber, Siqun Wang, Gregory Piskun, Ruben Dyall, Ping Song, Yoshizumi Takechi, Janko Nikolich-Zugich, Alan N. Houghton, Jonathan J. Lewis

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

DNA immunization has been shown to elicit both antibody and CTL responses against antigens expressed by infections organisms. Because CTL responses have been implicated in rejection of cancer, we investigated whether DNA immunization by particle bombardment using a gene gun could induce CTL responses that were capable of rejecting tumors in mice. DNA immunization by particle bombardment using genes encoding β-galactosidase and ovalbumin primed mice to generate CTLs in two genetic backgrounds (DBA/2 and C57BL/6 strains, respectively). DNA immunization was more potent in inducing CTLs than immunization with an optimized regimen of ovalbumin peptide plus immune adjuvant. Immunity induced by DNA immunization protected mice against s.c. challenge with tumors expressing the β-galactosidase antigen. Tumors were rejected even when DNA immunization was started 3 or 7 days after tumor challenge as tumors were becoming established. Tumor rejection required CD8+ T cells, confirming a role for CTLs in vivo. These studies show that DNA immunization by particle bombardment can efficiently induce CTL responses that are capable of rejecting even established tumors.

Original languageEnglish (US)
Pages (from-to)2191-2196
Number of pages6
JournalClinical Cancer Research
Volume3
Issue number12 I
StatePublished - Dec 1997
Externally publishedYes

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Immunization
T-Lymphocytes
Skin
DNA
Neoplasms
Galactosidases
Ovalbumin
Antigens
Firearms
Genes
Antibody Formation
Immunity
Peptides
Infection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ross, H. M., Weber, L. W., Wang, S., Piskun, G., Dyall, R., Song, P., ... Lewis, J. J. (1997). Priming for T-cell-mediated rejection of established tumors by cutaneous DNA immunization. Clinical Cancer Research, 3(12 I), 2191-2196.

Priming for T-cell-mediated rejection of established tumors by cutaneous DNA immunization. / Ross, Howard M.; Weber, Lawrence W.; Wang, Siqun; Piskun, Gregory; Dyall, Ruben; Song, Ping; Takechi, Yoshizumi; Nikolich-Zugich, Janko; Houghton, Alan N.; Lewis, Jonathan J.

In: Clinical Cancer Research, Vol. 3, No. 12 I, 12.1997, p. 2191-2196.

Research output: Contribution to journalArticle

Ross, HM, Weber, LW, Wang, S, Piskun, G, Dyall, R, Song, P, Takechi, Y, Nikolich-Zugich, J, Houghton, AN & Lewis, JJ 1997, 'Priming for T-cell-mediated rejection of established tumors by cutaneous DNA immunization', Clinical Cancer Research, vol. 3, no. 12 I, pp. 2191-2196.
Ross HM, Weber LW, Wang S, Piskun G, Dyall R, Song P et al. Priming for T-cell-mediated rejection of established tumors by cutaneous DNA immunization. Clinical Cancer Research. 1997 Dec;3(12 I):2191-2196.
Ross, Howard M. ; Weber, Lawrence W. ; Wang, Siqun ; Piskun, Gregory ; Dyall, Ruben ; Song, Ping ; Takechi, Yoshizumi ; Nikolich-Zugich, Janko ; Houghton, Alan N. ; Lewis, Jonathan J. / Priming for T-cell-mediated rejection of established tumors by cutaneous DNA immunization. In: Clinical Cancer Research. 1997 ; Vol. 3, No. 12 I. pp. 2191-2196.
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