Abstract
Pharmacotoxicology studies currently approved by the US FDA use only 2D static cell culture, which is not a good comparison to the complex structure and flow we find in vivo. 3D cell culture systems on microfluidic platforms could transform the way that we evaluate drug efficacy. Incorporation of gelatin electrospun nanofibers, containing submicron particles and cell binding peptides will drastically decrease time necessary for sufficient cell adhesion, proliferation and differentiation in organ-on-a-chip (OOC) research. Moreover, this scaffolding will improve realism of OOC by more closely mimicking native extracellular matrix (ECM). These improvements to the technology will provide results of cellular behavior in response to external stimuli, thus increasing realism in pharmaceutical testing. In this work we created an ECM-like substrate, which structurally and chemically mimics the basement structure healthy tissue, in a microfluidic system.
Original language | English (US) |
---|---|
Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
Publisher | Chemical and Biological Microsystems Society |
Pages | 760-762 |
Number of pages | 3 |
ISBN (Print) | 9780979806476 |
State | Published - 2014 |
Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Other
Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
---|---|
Country | United States |
City | San Antonio |
Period | 10/26/14 → 10/30/14 |
Keywords
- Derjaguin-Landau-Verwey-Overbeek theory
- Electrospinning
- Gelatin
- RGD ligand
ASJC Scopus subject areas
- Control and Systems Engineering