Probing mechanoregulation of neuronal differentiation by plasma lithography patterned elastomeric substrates

Ki Hwan Nam, Nima Jamilpour, Etienne Mfoumou, Fei Yue Wang, Donna Zhang, Pak Kin Wong

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cells sense and interpret mechanical cues, including cell-cell and cell-substrate interactions, in the microenvironment to collectively regulate various physiological functions. Understanding the influences of these mechanical factors on cell behavior is critical for fundamental cell biology and for the development of novel strategies in regenerative medicine. Here, we demonstrate plasma lithography patterning on elastomeric substrates for elucidating the influences of mechanical cues on neuronal differentiation and neuritogenesis. The neuroblastoma cells form neuronal spheres on plasma-treated regions, which geometrically confine the cells over two weeks. The elastic modulus of the elastomer is controlled simultaneously by the crosslinker concentration. The cell-substrate mechanical interactions are also investigated by controlling the size of neuronal spheres with different cell seeding densities. These physical cues are shown to modulate with the formation of focal adhesions, neurite outgrowth, and the morphology of neuroblastoma. By systematic adjustment of these cues, along with computational biomechanical analysis, we demonstrate the interrelated mechanoregulatory effects of substrate elasticity and cell size. Taken together, our results reveal that the neuronal differentiation and neuritogenesis of neuroblastoma cells are collectively regulated via the cell-substrate mechanical interactions.

Original languageEnglish (US)
Article number6965
JournalScientific Reports
Volume4
DOIs
StatePublished - 2014

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Cues
Neuroblastoma
Elastomers
Social Adjustment
elastomeric
Focal Adhesions
Regenerative Medicine
Elastic Modulus
Elasticity
Cell Size
Cell Communication
Cell Biology
Cell Count

ASJC Scopus subject areas

  • General

Cite this

Probing mechanoregulation of neuronal differentiation by plasma lithography patterned elastomeric substrates. / Nam, Ki Hwan; Jamilpour, Nima; Mfoumou, Etienne; Wang, Fei Yue; Zhang, Donna; Wong, Pak Kin.

In: Scientific Reports, Vol. 4, 6965, 2014.

Research output: Contribution to journalArticle

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