Production of tumor necrosis factor α by resident and activated murine macrophages

K. Tachibana, G. Chen, D. S. Huang, P. Scuderi, Ronald R Watson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Alveolar macrophages (AmΦs), resident peritoneal macrophages (RPmΦs), and thioglycolate-elicited peritoneal macrophages (TGPmΦs) were isolated from C57BL/6 mice and incubated with lipopolysaccharide (LPS), stimulated cell supernatant, or recombinant interferon γ (IFN-γ) for 24 h. Tumor necrosis factor (TNF) in cell-free supernatants was measured by enzyme-linked immunosorbent assay. AmoΦs incubated with 103 ng/ml LPS produced 50 times more TNF than RPmΦs and 5 times more than TGPmΦs, and LPS alone induced maximum TNF production by AmΦs. Stimulated cell supernatant or recombinant IFN-γ alone did not induce TNF production. A combination of LPS with stimulated cell supernatant or IFN-γ had only a limited synergistic effect on TNF production by AmΦs. However, both LPS and stimulated cell supernatant or recombinant IFN-γ induced maximum TNF production by RPmΦs and TGPmΦs. TGPmΦs showed greater sensitivity to LPS and stimulated cell supernatant or IFN-γ with regard to TNF production than the other macrophage populations investigated.

Original languageEnglish (US)
Pages (from-to)251-255
Number of pages5
JournalJournal of Leukocyte Biology
Volume51
Issue number3
StatePublished - 1992

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Peritoneal Macrophages
Tumor Necrosis Factor-alpha
Thioglycolates
Lipopolysaccharides
Macrophages
Interferons
Alveolar Macrophages
Inbred C57BL Mouse
Enzyme-Linked Immunosorbent Assay
Population

Keywords

  • macrophages
  • tumor necrosis factor

ASJC Scopus subject areas

  • Cell Biology

Cite this

Production of tumor necrosis factor α by resident and activated murine macrophages. / Tachibana, K.; Chen, G.; Huang, D. S.; Scuderi, P.; Watson, Ronald R.

In: Journal of Leukocyte Biology, Vol. 51, No. 3, 1992, p. 251-255.

Research output: Contribution to journalArticle

Tachibana, K, Chen, G, Huang, DS, Scuderi, P & Watson, RR 1992, 'Production of tumor necrosis factor α by resident and activated murine macrophages', Journal of Leukocyte Biology, vol. 51, no. 3, pp. 251-255.
Tachibana, K. ; Chen, G. ; Huang, D. S. ; Scuderi, P. ; Watson, Ronald R. / Production of tumor necrosis factor α by resident and activated murine macrophages. In: Journal of Leukocyte Biology. 1992 ; Vol. 51, No. 3. pp. 251-255.
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