Prohibitin as a novel target protein of luteinizing hormone in ovarian epithelial carcinogenesis

L. Jia, X. F. Yi, Z. B. Zhang, Z. P. Zhuang, J. Li, Setsuko K Chambers, B. H. Kong, Wenxin - Zheng

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The exact role of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in ovarian epithelial carcinoma (OEC) development has not been yet characterized. This prompted us to identify particular proteins to better understand the underlying mechanism. Total proteins from ovarian epithelial tumor (OET) cells treated with gonadotropins were analyzed by proteomics. Western blot and immunohistochemistry were used to validate the target protein (prohibitin) and to detect its expression in human ovarian tissue of serous tumors. As the results, prohibitin was found to be significantly up-regulated by LH, with a maximum of 2.5-fold increase at the concentration of 200 mIU/mL. The expression of prohibitin was steadily decreased from benign serous cystadenomas to borderline tumors and serous carcinomas (P < 0.0001). The difference between any two groups was significant (P < 0.001). Collectively, data from this study indicate that prohibitin is one LH-associated protein and it may be protective of ovarian cancer development and progression, supporting that LH may play an inhibitory role in ovarian tumorigenesis.

Original languageEnglish (US)
Pages (from-to)104-109
Number of pages6
JournalNeoplasma
Volume58
Issue number2
DOIs
StatePublished - 2011

Fingerprint

Luteinizing Hormone
Carcinogenesis
Proteins
Serous Cystadenoma
Carcinoma
Neoplasms
Follicle Stimulating Hormone
Gonadotropins
Proteomics
Ovarian Neoplasms
Western Blotting
Epithelial Cells
Immunohistochemistry
prohibitin

Keywords

  • Gonadotropin
  • Luteinizing hormone
  • Ovarian cancer
  • Prohibitin

ASJC Scopus subject areas

  • Cancer Research

Cite this

Prohibitin as a novel target protein of luteinizing hormone in ovarian epithelial carcinogenesis. / Jia, L.; Yi, X. F.; Zhang, Z. B.; Zhuang, Z. P.; Li, J.; Chambers, Setsuko K; Kong, B. H.; Zheng, Wenxin -.

In: Neoplasma, Vol. 58, No. 2, 2011, p. 104-109.

Research output: Contribution to journalArticle

Jia, L. ; Yi, X. F. ; Zhang, Z. B. ; Zhuang, Z. P. ; Li, J. ; Chambers, Setsuko K ; Kong, B. H. ; Zheng, Wenxin -. / Prohibitin as a novel target protein of luteinizing hormone in ovarian epithelial carcinogenesis. In: Neoplasma. 2011 ; Vol. 58, No. 2. pp. 104-109.
@article{fc7c964ed3fb429782527246ec43ec4e,
title = "Prohibitin as a novel target protein of luteinizing hormone in ovarian epithelial carcinogenesis",
abstract = "The exact role of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in ovarian epithelial carcinoma (OEC) development has not been yet characterized. This prompted us to identify particular proteins to better understand the underlying mechanism. Total proteins from ovarian epithelial tumor (OET) cells treated with gonadotropins were analyzed by proteomics. Western blot and immunohistochemistry were used to validate the target protein (prohibitin) and to detect its expression in human ovarian tissue of serous tumors. As the results, prohibitin was found to be significantly up-regulated by LH, with a maximum of 2.5-fold increase at the concentration of 200 mIU/mL. The expression of prohibitin was steadily decreased from benign serous cystadenomas to borderline tumors and serous carcinomas (P < 0.0001). The difference between any two groups was significant (P < 0.001). Collectively, data from this study indicate that prohibitin is one LH-associated protein and it may be protective of ovarian cancer development and progression, supporting that LH may play an inhibitory role in ovarian tumorigenesis.",
keywords = "Gonadotropin, Luteinizing hormone, Ovarian cancer, Prohibitin",
author = "L. Jia and Yi, {X. F.} and Zhang, {Z. B.} and Zhuang, {Z. P.} and J. Li and Chambers, {Setsuko K} and Kong, {B. H.} and Zheng, {Wenxin -}",
year = "2011",
doi = "10.4149/neo_2011_02_104",
language = "English (US)",
volume = "58",
pages = "104--109",
journal = "Neoplasma",
issn = "0028-2685",
publisher = "Vydavatel'stvo Slovenkej Akademie Vied/Veda Publishing House of the Slovak Academy of Sciences",
number = "2",

}

TY - JOUR

T1 - Prohibitin as a novel target protein of luteinizing hormone in ovarian epithelial carcinogenesis

AU - Jia, L.

AU - Yi, X. F.

AU - Zhang, Z. B.

AU - Zhuang, Z. P.

AU - Li, J.

AU - Chambers, Setsuko K

AU - Kong, B. H.

AU - Zheng, Wenxin -

PY - 2011

Y1 - 2011

N2 - The exact role of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in ovarian epithelial carcinoma (OEC) development has not been yet characterized. This prompted us to identify particular proteins to better understand the underlying mechanism. Total proteins from ovarian epithelial tumor (OET) cells treated with gonadotropins were analyzed by proteomics. Western blot and immunohistochemistry were used to validate the target protein (prohibitin) and to detect its expression in human ovarian tissue of serous tumors. As the results, prohibitin was found to be significantly up-regulated by LH, with a maximum of 2.5-fold increase at the concentration of 200 mIU/mL. The expression of prohibitin was steadily decreased from benign serous cystadenomas to borderline tumors and serous carcinomas (P < 0.0001). The difference between any two groups was significant (P < 0.001). Collectively, data from this study indicate that prohibitin is one LH-associated protein and it may be protective of ovarian cancer development and progression, supporting that LH may play an inhibitory role in ovarian tumorigenesis.

AB - The exact role of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in ovarian epithelial carcinoma (OEC) development has not been yet characterized. This prompted us to identify particular proteins to better understand the underlying mechanism. Total proteins from ovarian epithelial tumor (OET) cells treated with gonadotropins were analyzed by proteomics. Western blot and immunohistochemistry were used to validate the target protein (prohibitin) and to detect its expression in human ovarian tissue of serous tumors. As the results, prohibitin was found to be significantly up-regulated by LH, with a maximum of 2.5-fold increase at the concentration of 200 mIU/mL. The expression of prohibitin was steadily decreased from benign serous cystadenomas to borderline tumors and serous carcinomas (P < 0.0001). The difference between any two groups was significant (P < 0.001). Collectively, data from this study indicate that prohibitin is one LH-associated protein and it may be protective of ovarian cancer development and progression, supporting that LH may play an inhibitory role in ovarian tumorigenesis.

KW - Gonadotropin

KW - Luteinizing hormone

KW - Ovarian cancer

KW - Prohibitin

UR - http://www.scopus.com/inward/record.url?scp=79961156167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961156167&partnerID=8YFLogxK

U2 - 10.4149/neo_2011_02_104

DO - 10.4149/neo_2011_02_104

M3 - Article

C2 - 21275458

AN - SCOPUS:79961156167

VL - 58

SP - 104

EP - 109

JO - Neoplasma

JF - Neoplasma

SN - 0028-2685

IS - 2

ER -