Prohormone convertase and autocrine growth factor mRNAs are coexpressed in small cell lung carcinoma

M. P. Rounseville, Thomas P Davis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A hallmark of small cell lung carcinoma (SCLC) is the expression of autocrine growth factors such as neurotensin and gastrin-releasing peptide, which bind to cellular receptors and stimulate cell division. The biological activity of autocrine growth factors requires the concurrent expression of prohormone convertases that cleave the growth factors to their active form, suggesting the expression of these genes is linked in SCLCs. RNase protection assays were used to detect the expression of autocrine growth factor and prohormone convertase mRNAs in a panel of lung cancer cell lines. These mRNAs are coexpressed in SCLC and lung carcinoid cell lines, but not in normal lung epithelium or in non-small cell lung cancers. These findings, together with earlier results from our laboratory, suggest the expression of prohormone convertases has an important role in the development and maintenance of the SCLC phenotype and that autocrine growth factor and prohormone convertase genes respond to a common transcriptional activator in SCLC.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalJournal of Molecular Endocrinology
Volume25
Issue number1
StatePublished - 2000

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Proprotein Convertases
Small Cell Lung Carcinoma
Intercellular Signaling Peptides and Proteins
Messenger RNA
Gastrin-Releasing Peptide
Cell Line
Neurotensin
Lung
Carcinoid Tumor
Ribonucleases
Non-Small Cell Lung Carcinoma
Cell Division
Lung Neoplasms
Epithelium
Maintenance
Phenotype
Gene Expression
Genes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Prohormone convertase and autocrine growth factor mRNAs are coexpressed in small cell lung carcinoma. / Rounseville, M. P.; Davis, Thomas P.

In: Journal of Molecular Endocrinology, Vol. 25, No. 1, 2000, p. 121-128.

Research output: Contribution to journalArticle

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