Prolactin gene expression in human thymocytes

Kevin D. O'Neal, David W. Montgomery, Thao M. Truong, Li yuan Yu-Lee

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Recent evidence suggests that lymphocytes produce prolactin (PRL). Here, we report the cDNA cloning and expression of PRL from normal human thymocytes. Sequence analysis showed that the thymocyte cDNA encodes a 23 kDa protein which is identical to pituitary PRL. RNA blot analysis showed that the thymocyte PRL mRNA is approximately 170 nucleotides larger than the pituitary PRL message. PRL message was also detected in several non-pituitary human cell lines including Jurkat T, HeLa, and JEG cells. Furthermore, PRL gene expression in JEG cells was inhibited by glucocorticoid treatment. Our data support the hypothesis that PRL is a T cell-derived cytokine.

Original languageEnglish (US)
Pages (from-to)R19-R23
JournalMolecular and Cellular Endocrinology
Volume87
Issue number1-3
DOIs
StatePublished - Sep 1992

Keywords

  • Cytokine
  • Prolactin
  • Thymocyte
  • cDNA sequence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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