Prolactin gene expression in human thymocytes

Kevin D. O'Neal, David W Montgomery, Thao M. Truong, Li yuan Yu-Lee

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Recent evidence suggests that lymphocytes produce prolactin (PRL). Here, we report the cDNA cloning and expression of PRL from normal human thymocytes. Sequence analysis showed that the thymocyte cDNA encodes a 23 kDa protein which is identical to pituitary PRL. RNA blot analysis showed that the thymocyte PRL mRNA is approximately 170 nucleotides larger than the pituitary PRL message. PRL message was also detected in several non-pituitary human cell lines including Jurkat T, HeLa, and JEG cells. Furthermore, PRL gene expression in JEG cells was inhibited by glucocorticoid treatment. Our data support the hypothesis that PRL is a T cell-derived cytokine.

Original languageEnglish (US)
JournalMolecular and Cellular Endocrinology
Volume87
Issue number1-3
DOIs
StatePublished - 1992

Fingerprint

Thymocytes
Gene expression
Prolactin
Gene Expression
Complementary DNA
T-cells
Lymphocytes
Cloning
HeLa Cells
Glucocorticoids
Sequence Analysis
Organism Cloning
Nucleotides
Cells
RNA
Cytokines
T-Lymphocytes
Cell Line
Messenger RNA

Keywords

  • cDNA sequence
  • Cytokine
  • Prolactin
  • Thymocyte

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Prolactin gene expression in human thymocytes. / O'Neal, Kevin D.; Montgomery, David W; Truong, Thao M.; Yu-Lee, Li yuan.

In: Molecular and Cellular Endocrinology, Vol. 87, No. 1-3, 1992.

Research output: Contribution to journalArticle

O'Neal, Kevin D. ; Montgomery, David W ; Truong, Thao M. ; Yu-Lee, Li yuan. / Prolactin gene expression in human thymocytes. In: Molecular and Cellular Endocrinology. 1992 ; Vol. 87, No. 1-3.
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