Prolactin-provoked alterations of cytosolic, membrane, and nuclear protein kinase C following partial hepatectomy

Arthur R. Buckley, Paul D. Crowe, Patricia A. Bauman, Leigh A Neumayer, Hugh E. Laird, Diane Haddock Russell, Charles W. Putnam

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The adenohypophyseal polypeptide hormone prolactin is a potent liver mitogen, stimulating cell cycle progression, an effect that appears coupled to increasing protein kinase C activity in membrane and nuclear fractions. Here, we examine whether hepatocyte proliferation, stimulated by partial hepatectomy, is associated with altered serum prolactin or protein kinase C activation. Within 5-15 min of liver resection, serum prolactin concentrations elevate significantly. Protein kinase C activity in hepatic cytosol decreases significantly, and membrane and nuclear PKC activity increase by 30 min. Hypophysectomy prior to partial hepatectomy abrogates any effect of liver resection on protein kinase C activation in the hepatic remnant. Based upon these data, it is suggested that the rapid increase in serum prolactin seen after partial hepatectomy may be linked to protein kinase C activation, which in turn stimulates the hepatic proliferative response that is essential for hepatic regeneration.

Original languageEnglish (US)
Pages (from-to)1313-1319
Number of pages7
JournalDigestive Diseases and Sciences
Volume36
Issue number9
DOIs
StatePublished - Sep 1991

Fingerprint

Hepatectomy
Nuclear Proteins
Prolactin
Protein Kinase C
Membrane Proteins
Liver
Nuclear Envelope
Serum
Anterior Pituitary Hormones
Hypophysectomy
Peptide Hormones
Mitogens
Cytosol
Regeneration
Hepatocytes
Cell Cycle

Keywords

  • hypophysectomy
  • liver regeneration
  • nucleus
  • prolactin
  • protein kinase C

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Prolactin-provoked alterations of cytosolic, membrane, and nuclear protein kinase C following partial hepatectomy. / Buckley, Arthur R.; Crowe, Paul D.; Bauman, Patricia A.; Neumayer, Leigh A; Laird, Hugh E.; Russell, Diane Haddock; Putnam, Charles W.

In: Digestive Diseases and Sciences, Vol. 36, No. 9, 09.1991, p. 1313-1319.

Research output: Contribution to journalArticle

Buckley, Arthur R. ; Crowe, Paul D. ; Bauman, Patricia A. ; Neumayer, Leigh A ; Laird, Hugh E. ; Russell, Diane Haddock ; Putnam, Charles W. / Prolactin-provoked alterations of cytosolic, membrane, and nuclear protein kinase C following partial hepatectomy. In: Digestive Diseases and Sciences. 1991 ; Vol. 36, No. 9. pp. 1313-1319.
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