Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons

H. Laeng, David T. Harris, R. Schindler

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Abstract

Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is characterized by its dominant expression in heterokaryons [1] and by morphological differentiation with the formation of metachromatically staining granules similar to those of mast cells [2]. This suggests that the cellular control mechanisms involved in entry into proliferative quiescence and in morphological differentiation of cs 21-F cells may be analogous to those of normal mast cells and/or their precursors.

Original languageEnglish (US)
Pages (from-to)170-176
Number of pages7
JournalExperimental Cell Research
Volume158
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

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Mastocytoma
Mast Cells
Serum
S Phase
Horses
Cultured Cells
Cell Cycle
Staining and Labeling
Temperature

ASJC Scopus subject areas

  • Cell Biology

Cite this

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title = "Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons",
abstract = "Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10{\%} horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is characterized by its dominant expression in heterokaryons [1] and by morphological differentiation with the formation of metachromatically staining granules similar to those of mast cells [2]. This suggests that the cellular control mechanisms involved in entry into proliferative quiescence and in morphological differentiation of cs 21-F cells may be analogous to those of normal mast cells and/or their precursors.",
author = "H. Laeng and Harris, {David T.} and R. Schindler",
year = "1985",
doi = "10.1016/0014-4827(85)90441-0",
language = "English (US)",
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pages = "170--176",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
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T1 - Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons

AU - Laeng, H.

AU - Harris, David T.

AU - Schindler, R.

PY - 1985

Y1 - 1985

N2 - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is characterized by its dominant expression in heterokaryons [1] and by morphological differentiation with the formation of metachromatically staining granules similar to those of mast cells [2]. This suggests that the cellular control mechanisms involved in entry into proliferative quiescence and in morphological differentiation of cs 21-F cells may be analogous to those of normal mast cells and/or their precursors.

AB - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The quiescent state of normal mast cells thus resembles that of cold-sensitive (cs) mutant cells (21-F) of the undifferentiated P-815 mastocytoma: at the non-permissive temperature of 33 °C, the 21-F cells were found to enter a state of quiescence which is characterized by its dominant expression in heterokaryons [1] and by morphological differentiation with the formation of metachromatically staining granules similar to those of mast cells [2]. This suggests that the cellular control mechanisms involved in entry into proliferative quiescence and in morphological differentiation of cs 21-F cells may be analogous to those of normal mast cells and/or their precursors.

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