Proline-rich region of non-muscle myosin light chain kinase modulates kinase activity and endothelial cytoskeletal dynamics

Patrick Belvitch, Djanybek Adyshev, Venkateswaran R. Elangovan, Mary E. Brown, Caitlin Naureckas, Alicia N. Rizzo, Jessica H. Siegler, Joe GN Garcia, Steven M. Dudek

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Disruption of the pulmonary endothelial barrier and subsequent vascular leak is a hallmark of acute lung injury. Dynamic rearrangements in the endothelial cell (EC) peripheral membrane and underlying cytoskeleton are critical determinants of barrier function. The cytoskeletal effector protein non-muscle myosin light chain kinase (nmMLCK) and the actin-binding regulatory protein cortactin are important regulators of the endothelial barrier. In the present study we functionally characterize a proline-rich region of nmMLCK previously identified as the possible site of interaction between nmMLCK and cortactin. A mutant nmMLCK construct deficient in proline residues at the putative sites of cortactin binding (amino acids 973, 976, 1019, 1022) was generated. Co-immunoprecipitation studies in human lung EC transfected with wild-type or mutant nmMLCK demonstrated similar levels of cortactin interaction at baseline and after stimulation with the barrier-enhancing agonist, sphingosine 1-phosphate (S1P). In contrast, binding studies utilizing recombinant nmMLCK fragments containing the wild-type or proline-deficient sequence demonstrated a two-fold increase in cortactin binding (p. <. 0.01) to the mutant construct. Immunofluorescent microscopy revealed an increased stress fiber density in ECs expressing GFP-labeled mutant nmMLCK at baseline (p = 0.02) and after thrombin (p = 0.01) or S1P (p = 0.02) when compared to wild-type. Mutant nmMLCK demonstrated an increase in kinase activity in response to thrombin (p. <. 0.01). Kymographic analysis demonstrated an increased EC membrane retraction distance and velocity (p. <. 0.01) in response to the barrier disrupting agent thrombin in cells expressing the mutant vs. the wild-type nmMLCK construct. These results provide evidence that critical prolines within nmMLCK (amino acids 973, 976, 1019, 1022) regulate cytoskeletal and membrane events associated with pulmonary endothelial barrier function.

Original languageEnglish (US)
Pages (from-to)94-102
Number of pages9
JournalMicrovascular Research
Volume95
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Myosin-Light-Chain Kinase
Proline
Phosphotransferases
Cortactin
Endothelial cells
Thrombin
Endothelial Cells
Lung
Cell Membrane
Membranes
Amino Acids
Microfilament Proteins
Stress Fibers
Cytoskeletal Proteins
Acute Lung Injury
Cell membranes
Cytoskeleton
Immunoprecipitation
Blood Vessels
Actins

Keywords

  • ARDS
  • Barrier function
  • Cortactin
  • Cytoskeleton
  • Endothelium
  • Membrane dynamics
  • Non-muscle myosin light chain kinase
  • Stress fibers

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology
  • Medicine(all)

Cite this

Belvitch, P., Adyshev, D., Elangovan, V. R., Brown, M. E., Naureckas, C., Rizzo, A. N., ... Dudek, S. M. (2014). Proline-rich region of non-muscle myosin light chain kinase modulates kinase activity and endothelial cytoskeletal dynamics. Microvascular Research, 95(1), 94-102. https://doi.org/10.1016/j.mvr.2014.07.007

Proline-rich region of non-muscle myosin light chain kinase modulates kinase activity and endothelial cytoskeletal dynamics. / Belvitch, Patrick; Adyshev, Djanybek; Elangovan, Venkateswaran R.; Brown, Mary E.; Naureckas, Caitlin; Rizzo, Alicia N.; Siegler, Jessica H.; Garcia, Joe GN; Dudek, Steven M.

In: Microvascular Research, Vol. 95, No. 1, 2014, p. 94-102.

Research output: Contribution to journalArticle

Belvitch, P, Adyshev, D, Elangovan, VR, Brown, ME, Naureckas, C, Rizzo, AN, Siegler, JH, Garcia, JGN & Dudek, SM 2014, 'Proline-rich region of non-muscle myosin light chain kinase modulates kinase activity and endothelial cytoskeletal dynamics', Microvascular Research, vol. 95, no. 1, pp. 94-102. https://doi.org/10.1016/j.mvr.2014.07.007
Belvitch, Patrick ; Adyshev, Djanybek ; Elangovan, Venkateswaran R. ; Brown, Mary E. ; Naureckas, Caitlin ; Rizzo, Alicia N. ; Siegler, Jessica H. ; Garcia, Joe GN ; Dudek, Steven M. / Proline-rich region of non-muscle myosin light chain kinase modulates kinase activity and endothelial cytoskeletal dynamics. In: Microvascular Research. 2014 ; Vol. 95, No. 1. pp. 94-102.
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AU - Brown, Mary E.

AU - Naureckas, Caitlin

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AU - Garcia, Joe GN

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