Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements

David Twell, Judy Yamaguchi, Rod A Wing, James Ushiba, Sheila McCormick

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

We have investigated the functional organization and properties of cis regulatory elements in the promoter regions of two genes from tomato (LAT52 and LAT59) that are preferentially and coordinately expressed during pollen maturation. Promoter deletion analysis in transgenic plants demonstrated that only minimal (<200 bp) promoter proximal regions are required for developmentally regulated expression in pollen and in specific cell types of the sporophyte. Cis-acting regulatory regions of these two promoters and of a third pollen-expressed promoter (LAT56) were characterized in detail using a transient expression assay. We identified two upstream activator regions in the LAT52 promoter and further showed that a 19-bp segment from one of those regions enhanced expression of the heterologous CaMV35S promoter in pollen. Similarities in sequence between crucial cis elements provide evidence that shared regulatory elements are involved in the coordinate expression of the LAT genes during microsporogenesis.

Original languageEnglish (US)
Pages (from-to)496-507
Number of pages12
JournalGenes and Development
Volume5
Issue number3
StatePublished - 1991
Externally publishedYes

Fingerprint

Pollen
Genetic Promoter Regions
Genes
Plant Gametogenesis
Nucleic Acid Regulatory Sequences
Genetically Modified Plants
Lycopersicon esculentum
Gene Expression

Keywords

  • Cell-specific gene regulation
  • Enhancer
  • Male gametophyte
  • Tobacco
  • Tomato
  • Transcriptional control

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements. / Twell, David; Yamaguchi, Judy; Wing, Rod A; Ushiba, James; McCormick, Sheila.

In: Genes and Development, Vol. 5, No. 3, 1991, p. 496-507.

Research output: Contribution to journalArticle

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