Proposal of three-dimensional phase contrast holographic microscopy

Naoki Fukutake, Thomas D Milster

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We propose a three-dimensional phase contrast digital holographic microscopy. The object to be observed is a low-contrast transparent refractive index distribution sample, such as biological tissue. Low contrast phase objects are converted to high contrast images through the microscopy we propose. In order to gain high three-dimensional resolution, the direction of pump plane wave is scanned, and separate holographic images produced at each angle are acquired and decoded into complex amplitude in Fourier space. The three-dimensional image is reconstructed in a computer from all information acquired through the system. The resolution in the direction of the optical axis is increased by utilizing a Aπ configuration of objective lenzes.

Original languageEnglish (US)
Pages (from-to)12662-12679
Number of pages18
JournalOptics Express
Volume15
Issue number20
DOIs
StatePublished - Oct 1 2007

Fingerprint

phase contrast
proposals
microscopy
image contrast
high gain
plane waves
refractivity
pumps
configurations

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Proposal of three-dimensional phase contrast holographic microscopy. / Fukutake, Naoki; Milster, Thomas D.

In: Optics Express, Vol. 15, No. 20, 01.10.2007, p. 12662-12679.

Research output: Contribution to journalArticle

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