Propylparaben inhibits mouse cultured antral follicle growth, alters steroidogenesis, and upregulates levels of cell-cycle and apoptosis regulators

Arnon Gal, Kristene Gedye, Zelieann R Craig, Ayelet Ziv-Gal

Research output: Contribution to journalArticle

Abstract

Propylparaben is prevalently used in cosmetics, pharmaceuticals, and foods; yet, its direct effects on the mammalian ovary are unknown. We investigated the direct effects of propylparaben on the growth and steroidogenic function of mouse antral follicles. Antral follicles were isolated from the ovaries of Swiss mice (age: 32–42 days) and cultured in media with dimethylsulfoxide vehicle control or propylparaben (0.01–100 μg/mL) for 24–72 h. Follicle diameter was measured every 24 h to assess growth. Follicles and media were collected at 24 and 72 h for gene expression and hormone measurements. Propylparaben (100 μg/mL) significantly inhibited follicle growth (48–72 h). Further, propylparaben exposure increased expression of cell cycle regulators (Cdk4, Cdkn1a), an apoptotic factor (Bax), and a key steroidogenic regulator (Star). In media, propylparaben decreased accumulation of dehydroepiandrosterone-sulfate, but increased testosterone and 17β-estradiol. Overall, our findings suggest that propylparaben disrupts antral follicle growth and steroidogenic function by altering the cell-cycle, apoptosis, and steroidogenesis pathways.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalReproductive Toxicology
Volume89
DOIs
StatePublished - Oct 1 2019

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Cell Cycle
Up-Regulation
Cells
Apoptosis
Growth
Ovary
Dehydroepiandrosterone Sulfate
Cosmetics
Dimethyl Sulfoxide
Antral
propylparaben
Gene expression
Stars
Testosterone
Estradiol
Hormones
Gene Expression
Food
Pharmaceutical Preparations

Keywords

  • Apoptosis
  • Cell-cycle
  • Cosmetics
  • Ovary
  • Paraben
  • Steroidogenesis

ASJC Scopus subject areas

  • Toxicology

Cite this

Propylparaben inhibits mouse cultured antral follicle growth, alters steroidogenesis, and upregulates levels of cell-cycle and apoptosis regulators. / Gal, Arnon; Gedye, Kristene; Craig, Zelieann R; Ziv-Gal, Ayelet.

In: Reproductive Toxicology, Vol. 89, 01.10.2019, p. 100-106.

Research output: Contribution to journalArticle

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