Prostaglandin F-induced calcium transient in ovine large luteal cells: I. Alterations in cytosolic-free calcium levels and calcium flux

J. A. Wegner, R. Martinez-Zaguilan, M. E. Wise, R. J. Gillies, Patricia B Hoyer

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The effect of prostaglandin F (PGF) on cytosolic calcium homeostasis was studied in suspensions of ovine large or small luteal cells from superovulated ewes. In large cells loaded with fura-2 (AM), resting cytosolic-free calcium ([Ca2+]i) was 62 ± 5 nM (Hanks' medium, pH 7.15), and PGF (0.5 μM) induced a rapid transient increase in [Ca2+]i to 152 ± 6 nM, which then decreased to 97 ± 6 nM within 3 min and remained at this level for the remainder of the treatment period (10-20 min). PGF did not alter intracellular pH (pHi) in cells loaded with snarf-1 (AM) (pHi indicator). The transient nature of the [Ca2+]i increase was due, at least in part, to the ability of PGF to stimulate (P < 0.05) 45Ca2+ efflux. In small cells, resting [Ca2+]i was 57 ± 5 nM, and no change in [Ca2+]i levels or PHi occurred with the addition of PGF. PGF also did not affect 45Ca2+ efflux in small cells. Calcium uptake was not significantly altered by PGF in large or small cells. Data from kinetic analysis of the calcium transient was best fit to a two-compartment model consisting of a rapidly effluxing compartment and a slowly effluxing compartment. The size and rate constants were 62 ± 10 nM and 3.6 ± 1 min-1, respectively, for the rapidly effluxing compartment and 140 ± 9 nM and 0.02 ± 0.002 min-1, respectively, for the slowly effluxing compartment. These results provide evidence for a direct effect of PGF specifically on the ovine large luteal cell that involves alterations in [Ca2+]i and calcium flux. This effect is likely to be involved in intracellular mediation of the signal for luteal regression.

Original languageEnglish (US)
Pages (from-to)3029-3037
Number of pages9
JournalEndocrinology
Volume127
Issue number6
StatePublished - Dec 1990

Fingerprint

Luteal Cells
Dinoprost
Sheep
Calcium
Luteolysis
Fura-2
Suspensions
Homeostasis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Prostaglandin F-induced calcium transient in ovine large luteal cells : I. Alterations in cytosolic-free calcium levels and calcium flux. / Wegner, J. A.; Martinez-Zaguilan, R.; Wise, M. E.; Gillies, R. J.; Hoyer, Patricia B.

In: Endocrinology, Vol. 127, No. 6, 12.1990, p. 3029-3037.

Research output: Contribution to journalArticle

Wegner, J. A. ; Martinez-Zaguilan, R. ; Wise, M. E. ; Gillies, R. J. ; Hoyer, Patricia B. / Prostaglandin F-induced calcium transient in ovine large luteal cells : I. Alterations in cytosolic-free calcium levels and calcium flux. In: Endocrinology. 1990 ; Vol. 127, No. 6. pp. 3029-3037.
@article{864235cba50d4cb79649a4830bffae7d,
title = "Prostaglandin F2α-induced calcium transient in ovine large luteal cells: I. Alterations in cytosolic-free calcium levels and calcium flux",
abstract = "The effect of prostaglandin F2α (PGF2α) on cytosolic calcium homeostasis was studied in suspensions of ovine large or small luteal cells from superovulated ewes. In large cells loaded with fura-2 (AM), resting cytosolic-free calcium ([Ca2+]i) was 62 ± 5 nM (Hanks' medium, pH 7.15), and PGF2α (0.5 μM) induced a rapid transient increase in [Ca2+]i to 152 ± 6 nM, which then decreased to 97 ± 6 nM within 3 min and remained at this level for the remainder of the treatment period (10-20 min). PGF2α did not alter intracellular pH (pHi) in cells loaded with snarf-1 (AM) (pHi indicator). The transient nature of the [Ca2+]i increase was due, at least in part, to the ability of PGF2α to stimulate (P < 0.05) 45Ca2+ efflux. In small cells, resting [Ca2+]i was 57 ± 5 nM, and no change in [Ca2+]i levels or PHi occurred with the addition of PGF2α. PGF2α also did not affect 45Ca2+ efflux in small cells. Calcium uptake was not significantly altered by PGF2α in large or small cells. Data from kinetic analysis of the calcium transient was best fit to a two-compartment model consisting of a rapidly effluxing compartment and a slowly effluxing compartment. The size and rate constants were 62 ± 10 nM and 3.6 ± 1 min-1, respectively, for the rapidly effluxing compartment and 140 ± 9 nM and 0.02 ± 0.002 min-1, respectively, for the slowly effluxing compartment. These results provide evidence for a direct effect of PGF2α specifically on the ovine large luteal cell that involves alterations in [Ca2+]i and calcium flux. This effect is likely to be involved in intracellular mediation of the signal for luteal regression.",
author = "Wegner, {J. A.} and R. Martinez-Zaguilan and Wise, {M. E.} and Gillies, {R. J.} and Hoyer, {Patricia B}",
year = "1990",
month = "12",
language = "English (US)",
volume = "127",
pages = "3029--3037",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Prostaglandin F2α-induced calcium transient in ovine large luteal cells

T2 - I. Alterations in cytosolic-free calcium levels and calcium flux

AU - Wegner, J. A.

AU - Martinez-Zaguilan, R.

AU - Wise, M. E.

AU - Gillies, R. J.

AU - Hoyer, Patricia B

PY - 1990/12

Y1 - 1990/12

N2 - The effect of prostaglandin F2α (PGF2α) on cytosolic calcium homeostasis was studied in suspensions of ovine large or small luteal cells from superovulated ewes. In large cells loaded with fura-2 (AM), resting cytosolic-free calcium ([Ca2+]i) was 62 ± 5 nM (Hanks' medium, pH 7.15), and PGF2α (0.5 μM) induced a rapid transient increase in [Ca2+]i to 152 ± 6 nM, which then decreased to 97 ± 6 nM within 3 min and remained at this level for the remainder of the treatment period (10-20 min). PGF2α did not alter intracellular pH (pHi) in cells loaded with snarf-1 (AM) (pHi indicator). The transient nature of the [Ca2+]i increase was due, at least in part, to the ability of PGF2α to stimulate (P < 0.05) 45Ca2+ efflux. In small cells, resting [Ca2+]i was 57 ± 5 nM, and no change in [Ca2+]i levels or PHi occurred with the addition of PGF2α. PGF2α also did not affect 45Ca2+ efflux in small cells. Calcium uptake was not significantly altered by PGF2α in large or small cells. Data from kinetic analysis of the calcium transient was best fit to a two-compartment model consisting of a rapidly effluxing compartment and a slowly effluxing compartment. The size and rate constants were 62 ± 10 nM and 3.6 ± 1 min-1, respectively, for the rapidly effluxing compartment and 140 ± 9 nM and 0.02 ± 0.002 min-1, respectively, for the slowly effluxing compartment. These results provide evidence for a direct effect of PGF2α specifically on the ovine large luteal cell that involves alterations in [Ca2+]i and calcium flux. This effect is likely to be involved in intracellular mediation of the signal for luteal regression.

AB - The effect of prostaglandin F2α (PGF2α) on cytosolic calcium homeostasis was studied in suspensions of ovine large or small luteal cells from superovulated ewes. In large cells loaded with fura-2 (AM), resting cytosolic-free calcium ([Ca2+]i) was 62 ± 5 nM (Hanks' medium, pH 7.15), and PGF2α (0.5 μM) induced a rapid transient increase in [Ca2+]i to 152 ± 6 nM, which then decreased to 97 ± 6 nM within 3 min and remained at this level for the remainder of the treatment period (10-20 min). PGF2α did not alter intracellular pH (pHi) in cells loaded with snarf-1 (AM) (pHi indicator). The transient nature of the [Ca2+]i increase was due, at least in part, to the ability of PGF2α to stimulate (P < 0.05) 45Ca2+ efflux. In small cells, resting [Ca2+]i was 57 ± 5 nM, and no change in [Ca2+]i levels or PHi occurred with the addition of PGF2α. PGF2α also did not affect 45Ca2+ efflux in small cells. Calcium uptake was not significantly altered by PGF2α in large or small cells. Data from kinetic analysis of the calcium transient was best fit to a two-compartment model consisting of a rapidly effluxing compartment and a slowly effluxing compartment. The size and rate constants were 62 ± 10 nM and 3.6 ± 1 min-1, respectively, for the rapidly effluxing compartment and 140 ± 9 nM and 0.02 ± 0.002 min-1, respectively, for the slowly effluxing compartment. These results provide evidence for a direct effect of PGF2α specifically on the ovine large luteal cell that involves alterations in [Ca2+]i and calcium flux. This effect is likely to be involved in intracellular mediation of the signal for luteal regression.

UR - http://www.scopus.com/inward/record.url?scp=0025572728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025572728&partnerID=8YFLogxK

M3 - Article

C2 - 2249641

AN - SCOPUS:0025572728

VL - 127

SP - 3029

EP - 3037

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -