Prostate-specific antigen immunoassays on the BioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The BioCD is a spinning-disc interferometric biosensor on which antibodies are immobilized to capture target antigens from biological samples. In this work, BioCDs measured the interferometric response to prostate-specific antigen (PSA). The ideal detection limit for PSA was determined using a BioCD with 12,500 printed target antibody spots with a corresponding number of reference protein spots. Statistical analysis projects the detection limit of PSA as a function of the number of spots included in the average. When approximately 10,000 spot pairs were averaged, the 3σ detection limit was 60 pg/ml in a 2 mg/ml simple protein background. A standard format for BioCD immunoassays uses 96 wells with 32 target spots paired with reference spots. In serum, the detection limit for this format was 1 ng/ml in 3:1 diluted female human serum using a sandwich assay with a nonfluorescent mass tag.

Original languageEnglish (US)
Pages (from-to)1151-1156
Number of pages6
JournalAnalytical and bioanalytical chemistry
Volume393
Issue number4
DOIs
StatePublished - Feb 1 2009

Keywords

  • Antibody microarray
  • BioCD
  • Label-free
  • Optical biosensor
  • Prostate cancer
  • Prostate-specific antigen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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