Prostate-specific antigen immunoassays on the BioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The BioCD is a spinning-disc interferometric biosensor on which antibodies are immobilized to capture target antigens from biological samples. In this work, BioCDs measured the interferometric response to prostate-specific antigen (PSA). The ideal detection limit for PSA was determined using a BioCD with 12,500 printed target antibody spots with a corresponding number of reference protein spots. Statistical analysis projects the detection limit of PSA as a function of the number of spots included in the average. When approximately 10,000 spot pairs were averaged, the 3σ detection limit was 60 pg/ml in a 2 mg/ml simple protein background. A standard format for BioCD immunoassays uses 96 wells with 32 target spots paired with reference spots. In serum, the detection limit for this format was 1 ng/ml in 3:1 diluted female human serum using a sandwich assay with a nonfluorescent mass tag.

Original languageEnglish (US)
Pages (from-to)1151-1156
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume393
Issue number4
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Prostate-Specific Antigen
Immunoassay
Limit of Detection
Immobilized Antibodies
Biosensors
Assays
Statistical methods
Proteins
Biosensing Techniques
Serum
Antigens
Antibodies

Keywords

  • Antibody microarray
  • BioCD
  • Label-free
  • Optical biosensor
  • Prostate cancer
  • Prostate-specific antigen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Prostate-specific antigen immunoassays on the BioCD. / Wang, Xuefeng; Zhao, Ming; Nolte, David D.

In: Analytical and Bioanalytical Chemistry, Vol. 393, No. 4, 02.2009, p. 1151-1156.

Research output: Contribution to journalArticle

Wang, Xuefeng ; Zhao, Ming ; Nolte, David D. / Prostate-specific antigen immunoassays on the BioCD. In: Analytical and Bioanalytical Chemistry. 2009 ; Vol. 393, No. 4. pp. 1151-1156.
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