Prostate tumor cell invasion: A comparison of orthotopic and ectopic models

J. D. Knox, C. F. Mack, W. C. Powell, G. T. Bowden, Raymond B Nagle

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Interest in orthotopic models has been generated by recent reports of increased invasive and metastatic potential demonstrated by tumor cell lines following injection into their tissue of origin rather than subcutaneously. We have previously demonstrated that transfection of the tumorigenic human prostate cell line, Du-145, with the metalloproteinase matrilysin increased its ability to invade the diaphragm following an intraperitoneal injection. In this study we compare the invasive and metastatic behavior of transfected Du-145 cell lines injected into the dorsal lateral lobe of the prostate to that observed when they are injected intraperitoneally. Immunohistochemistry was used to examine 37 orthotopically injected severe combined immunodeficient mice for local invasion and metastatic lesions. In addition, the effect of injection site on the level of expression of four genes thought to influence the invasiveness of tumor cells (matrilysin, stromelysin, TIMP-1, and TIMP-2), was determined by northern analysis of orthotopic and subcutaneous tumor tissue. The results demonstrate that the level of mRNA expression of the genes examined was similar at the two sites of injection and that the invasive properties of Du-145 cells following orthotopic implantation were comparable to that observed on the diaphragm following intraperitoneal injection. The advantages of the diaphragm invasion model are: less procedure-related mortality, ease of cell delivery, and provision of an easily orientated structure in which the earliest penetration of a basal lamina can be observed.

Original languageEnglish (US)
Pages (from-to)325-331
Number of pages7
JournalInvasion and Metastasis
Volume13
Issue number6
StatePublished - 1993

Fingerprint

Diaphragm
Matrix Metalloproteinase 7
Prostate
Intraperitoneal Injections
Injections
Gene Expression
Tissue Inhibitor of Metalloproteinase-2
Cell Line
Matrix Metalloproteinase 3
Neoplasms
Tissue Inhibitor of Metalloproteinase-1
Aptitude
SCID Mice
Subcutaneous Tissue
Metalloproteases
Tumor Cell Line
Basement Membrane
Transfection
Immunohistochemistry
Messenger RNA

Keywords

  • Invasion
  • Metastasis
  • Orthotopic model
  • Prostate

ASJC Scopus subject areas

  • Cancer Research

Cite this

Knox, J. D., Mack, C. F., Powell, W. C., Bowden, G. T., & Nagle, R. B. (1993). Prostate tumor cell invasion: A comparison of orthotopic and ectopic models. Invasion and Metastasis, 13(6), 325-331.

Prostate tumor cell invasion : A comparison of orthotopic and ectopic models. / Knox, J. D.; Mack, C. F.; Powell, W. C.; Bowden, G. T.; Nagle, Raymond B.

In: Invasion and Metastasis, Vol. 13, No. 6, 1993, p. 325-331.

Research output: Contribution to journalArticle

Knox, JD, Mack, CF, Powell, WC, Bowden, GT & Nagle, RB 1993, 'Prostate tumor cell invasion: A comparison of orthotopic and ectopic models', Invasion and Metastasis, vol. 13, no. 6, pp. 325-331.
Knox, J. D. ; Mack, C. F. ; Powell, W. C. ; Bowden, G. T. ; Nagle, Raymond B. / Prostate tumor cell invasion : A comparison of orthotopic and ectopic models. In: Invasion and Metastasis. 1993 ; Vol. 13, No. 6. pp. 325-331.
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