Protease inhibitors of Manduca sexta expressed in transgenic cotton

John C. Thomas, Deanna G. Adams, Veronique D. Keppenne, Catherine C. Wasmann, Judith K Brown, Michael R. Kanost, Hans J. Bohnert

Research output: Contribution to journalArticle

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Abstract

To explore the effectiveness of insect derived protease inhibitors in protecting plants against insect feeding, anti-trypsin, anti-chymotrypsin and anti-elastase protease inhibitor (PI) genes from Manduca sexta L. were expressed in transgenic cotton (Gossypium hirsutum L.). From 198 independent transformants, 35 elite lines were further analyzed. Under the control of the 35S promoter of CaMV, PI accumulated to approximately 0.1% of total protein, depending on the tissue analyzed. Using cell-flow cytometry, DNA content/ nuclei of transgenic and non-transformed cotton were identical. On cotton plants expressing PIs, fecundity of Bemisia tabaci (Genn.), the sweetpotato whitefly, was reduced compared to controls. Expression of these protease inhibitors may reduce the developmental rate of B. tabaci and other insects, and provide a strategy for cotton protection.

Original languageEnglish (US)
Pages (from-to)758-762
Number of pages5
JournalPlant Cell Reports
Volume14
Issue number12
DOIs
StatePublished - Oct 1995

Fingerprint

Manduca sexta
proteinase inhibitors
Bemisia tabaci
cotton
genetically modified organisms
insects
elastase
chymotrypsin
Gossypium hirsutum
trypsin
flow cytometry
fecundity
promoter regions
DNA
genes
proteins
cells

Keywords

  • Plant transformation
  • Protease inhibitor

ASJC Scopus subject areas

  • Plant Science

Cite this

Thomas, J. C., Adams, D. G., Keppenne, V. D., Wasmann, C. C., Brown, J. K., Kanost, M. R., & Bohnert, H. J. (1995). Protease inhibitors of Manduca sexta expressed in transgenic cotton. Plant Cell Reports, 14(12), 758-762. https://doi.org/10.1007/BF00232917

Protease inhibitors of Manduca sexta expressed in transgenic cotton. / Thomas, John C.; Adams, Deanna G.; Keppenne, Veronique D.; Wasmann, Catherine C.; Brown, Judith K; Kanost, Michael R.; Bohnert, Hans J.

In: Plant Cell Reports, Vol. 14, No. 12, 10.1995, p. 758-762.

Research output: Contribution to journalArticle

Thomas, JC, Adams, DG, Keppenne, VD, Wasmann, CC, Brown, JK, Kanost, MR & Bohnert, HJ 1995, 'Protease inhibitors of Manduca sexta expressed in transgenic cotton', Plant Cell Reports, vol. 14, no. 12, pp. 758-762. https://doi.org/10.1007/BF00232917
Thomas JC, Adams DG, Keppenne VD, Wasmann CC, Brown JK, Kanost MR et al. Protease inhibitors of Manduca sexta expressed in transgenic cotton. Plant Cell Reports. 1995 Oct;14(12):758-762. https://doi.org/10.1007/BF00232917
Thomas, John C. ; Adams, Deanna G. ; Keppenne, Veronique D. ; Wasmann, Catherine C. ; Brown, Judith K ; Kanost, Michael R. ; Bohnert, Hans J. / Protease inhibitors of Manduca sexta expressed in transgenic cotton. In: Plant Cell Reports. 1995 ; Vol. 14, No. 12. pp. 758-762.
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