Protease inhibitors suppress in vitro growth of human small cell lung cancer

David A. Clark, Robert Day, Nabil Seidah, Terry W. Moody, Frank Cuttitta, Thomas P Davis

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The effect of the protease inhibitors Bowman Birk inhibitor (BBI) and aprotinin on the in vitro clonal growth of two human small cell lung cancer (SCLC) cell lines was investigated. In addition, the effect of BBI on the growth factor processing of proGRP by SCLC cells and on mRNA levels for prohormone convertase 1 and 2 (PC1 and PC2) in SCLC cells was examined. The protease inhibitors BBI and aprotinin significantly decreased growth in both SCLC cell lines studied. In NCI-H345 cells, BBI appears to inhibit the processing of proGRP to GRP, as indicated by Western blot analysis. NCI-H345 cells, when treated with BBI (100 μg/ml), also showed highly significant decreases of mRNA for PC1 and PC2 of about 50%. These data suggest that proteases serve an important role in the growth regulation of SCLC and that inhibitors of these proteases may be potent suppressors of SCLC growth at the level of the gene.

Original languageEnglish (US)
Pages (from-to)1021-1028
Number of pages8
JournalPeptides
Volume14
Issue number5
DOIs
StatePublished - 1993

Fingerprint

Small Cell Lung Carcinoma
Protease Inhibitors
Cells
Growth
Aprotinin
Proprotein Convertase 2
Proprotein Convertase 1
Messenger RNA
Cell Line
Processing
Intercellular Signaling Peptides and Proteins
Peptide Hydrolases
Genes
In Vitro Techniques
Western Blotting

Keywords

  • Gastrin-releasing peptide
  • Growth regulation
  • Peptide processing
  • Prohormone convertase 1 and 2
  • Protease inhibitors
  • Small cell lung cancer

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Protease inhibitors suppress in vitro growth of human small cell lung cancer. / Clark, David A.; Day, Robert; Seidah, Nabil; Moody, Terry W.; Cuttitta, Frank; Davis, Thomas P.

In: Peptides, Vol. 14, No. 5, 1993, p. 1021-1028.

Research output: Contribution to journalArticle

Clark, DA, Day, R, Seidah, N, Moody, TW, Cuttitta, F & Davis, TP 1993, 'Protease inhibitors suppress in vitro growth of human small cell lung cancer', Peptides, vol. 14, no. 5, pp. 1021-1028. https://doi.org/10.1016/0196-9781(93)90081-Q
Clark, David A. ; Day, Robert ; Seidah, Nabil ; Moody, Terry W. ; Cuttitta, Frank ; Davis, Thomas P. / Protease inhibitors suppress in vitro growth of human small cell lung cancer. In: Peptides. 1993 ; Vol. 14, No. 5. pp. 1021-1028.
@article{51dbdf9d05e9460284bad7da7566595f,
title = "Protease inhibitors suppress in vitro growth of human small cell lung cancer",
abstract = "The effect of the protease inhibitors Bowman Birk inhibitor (BBI) and aprotinin on the in vitro clonal growth of two human small cell lung cancer (SCLC) cell lines was investigated. In addition, the effect of BBI on the growth factor processing of proGRP by SCLC cells and on mRNA levels for prohormone convertase 1 and 2 (PC1 and PC2) in SCLC cells was examined. The protease inhibitors BBI and aprotinin significantly decreased growth in both SCLC cell lines studied. In NCI-H345 cells, BBI appears to inhibit the processing of proGRP to GRP, as indicated by Western blot analysis. NCI-H345 cells, when treated with BBI (100 μg/ml), also showed highly significant decreases of mRNA for PC1 and PC2 of about 50{\%}. These data suggest that proteases serve an important role in the growth regulation of SCLC and that inhibitors of these proteases may be potent suppressors of SCLC growth at the level of the gene.",
keywords = "Gastrin-releasing peptide, Growth regulation, Peptide processing, Prohormone convertase 1 and 2, Protease inhibitors, Small cell lung cancer",
author = "Clark, {David A.} and Robert Day and Nabil Seidah and Moody, {Terry W.} and Frank Cuttitta and Davis, {Thomas P}",
year = "1993",
doi = "10.1016/0196-9781(93)90081-Q",
language = "English (US)",
volume = "14",
pages = "1021--1028",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Protease inhibitors suppress in vitro growth of human small cell lung cancer

AU - Clark, David A.

AU - Day, Robert

AU - Seidah, Nabil

AU - Moody, Terry W.

AU - Cuttitta, Frank

AU - Davis, Thomas P

PY - 1993

Y1 - 1993

N2 - The effect of the protease inhibitors Bowman Birk inhibitor (BBI) and aprotinin on the in vitro clonal growth of two human small cell lung cancer (SCLC) cell lines was investigated. In addition, the effect of BBI on the growth factor processing of proGRP by SCLC cells and on mRNA levels for prohormone convertase 1 and 2 (PC1 and PC2) in SCLC cells was examined. The protease inhibitors BBI and aprotinin significantly decreased growth in both SCLC cell lines studied. In NCI-H345 cells, BBI appears to inhibit the processing of proGRP to GRP, as indicated by Western blot analysis. NCI-H345 cells, when treated with BBI (100 μg/ml), also showed highly significant decreases of mRNA for PC1 and PC2 of about 50%. These data suggest that proteases serve an important role in the growth regulation of SCLC and that inhibitors of these proteases may be potent suppressors of SCLC growth at the level of the gene.

AB - The effect of the protease inhibitors Bowman Birk inhibitor (BBI) and aprotinin on the in vitro clonal growth of two human small cell lung cancer (SCLC) cell lines was investigated. In addition, the effect of BBI on the growth factor processing of proGRP by SCLC cells and on mRNA levels for prohormone convertase 1 and 2 (PC1 and PC2) in SCLC cells was examined. The protease inhibitors BBI and aprotinin significantly decreased growth in both SCLC cell lines studied. In NCI-H345 cells, BBI appears to inhibit the processing of proGRP to GRP, as indicated by Western blot analysis. NCI-H345 cells, when treated with BBI (100 μg/ml), also showed highly significant decreases of mRNA for PC1 and PC2 of about 50%. These data suggest that proteases serve an important role in the growth regulation of SCLC and that inhibitors of these proteases may be potent suppressors of SCLC growth at the level of the gene.

KW - Gastrin-releasing peptide

KW - Growth regulation

KW - Peptide processing

KW - Prohormone convertase 1 and 2

KW - Protease inhibitors

KW - Small cell lung cancer

UR - http://www.scopus.com/inward/record.url?scp=0027500739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027500739&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(93)90081-Q

DO - 10.1016/0196-9781(93)90081-Q

M3 - Article

VL - 14

SP - 1021

EP - 1028

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 5

ER -