Protection against hypoxia-induced increase in blood-brain barrier permeability: Role of tight junction proteins and NFκB

Rachel C. Brown, Karen S. Mark, Richard D. Egleton, Jason D. Huber, Amanda R. Burroughs, Thomas P. Davis

Research output: Contribution to journalReview articlepeer-review

84 Scopus citations

Abstract

Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight junction protein expression, and studied potential mechanisms of action. We found that C6-glioma-conditioned media, but not rat astrocyte-conditioned media, protected against an increase in permeability induced by exposure to 1% oxygen for 24 hours. This hypoxic stress caused an increase in the expression of tight junction proteins claudin-1 and actin, particularly in cells treated with C6-conditioned media. We found that C6-conditioned media has a significantly higher level of both basic fibroblast growth factor and vascular endothelial growth factor. Treatment with C6-conditioned. media for 1 or 3 days protects against hypoxia-induced permeability increases, and this protective effect may be mediated by signal transduction pathways terminating at the transcription factor NFκB.

Original languageEnglish (US)
Pages (from-to)693-700
Number of pages8
JournalJournal of Cell Science
Volume116
Issue number4
DOIs
StatePublished - Feb 15 2003

Keywords

  • Actin
  • Basic fibroblast growth factor
  • Claudin-1
  • Hypoxic stress
  • NFκB
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cell Biology

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