Protective effects of dietary curcumin in mouse model of chemically induced colitis are strain dependent

Claire B Larmonier, Jennifer K. Uno, Nicolas Larmonier, Anna J. Midura, Barbara Timmermann, Fayez K Ghishan, Pawel R Kiela

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Curcumin (diferulolylmethane) has been shown to have a protective role in mouse models of inflammatory bowel diseases (IBD) and to reduce the relapse rate in human ulcerative colitis (UC), thus making it a potentially viable supportive treatment option. Trinitrobenzene sulfonic acid (TNBS) colitis in NKT-deficient SJL/J mice has been described as Th1-mediated inflammation, whereas BALB/c mice are believed to exhibit a mixed Th1/Th2 response. Methods: We therefore investigated the effect of dietary curcumin in colitis induced in these 2 strains. Results: In the BALB/c mice, curcumin significantly increased survival, prevented weight loss, and normalized disease activity. In the SJL/J mice, curcumin demonstrated no protective effects. Genomewide microarray analysis of colonic gene expression was employed to define the differential effect of curcumin in these 2 strains. This analysis not only confirmed the disparate responses of the 2 strains to curcumin but also indicated different responses to TNBS. Curcumin inhibited proliferation of splenocytes from naive BALB/c mice but not SJL/J mice when nonspecifically stimulated in vitro with concanavalin A (ConA). Proliferation of CD4+ splenocytes was inhibited in both strains, albeit with about a 2-fold higher IC50 in SJL/J mice. Secretion of IL-4 and IL-5 by CD4+ lymphocytes of BALB/c mice but not SJL/J mice was significantly augmented by ConA and reduced to control levels by curcumin. Conclusions: The efficacy of dietary curcumin in TNBS colitis varies in BALB/c and SJL/J mouse strains. Although the exact mechanism underlying these differences is unclear, the results suggest that the therapeutic value of dietary curcumin may differ depending on the nature of immune dysregulation in IBD.

Original languageEnglish (US)
Pages (from-to)780-793
Number of pages14
JournalInflammatory Bowel Diseases
Volume14
Issue number6
DOIs
StatePublished - Jun 2008

Fingerprint

Curcumin
Colitis
Trinitrobenzenes
Sulfonic Acids
Concanavalin A
Inflammatory Bowel Diseases
Interleukin-5
Microarray Analysis
Ulcerative Colitis
Interleukin-4
Inhibitory Concentration 50
Weight Loss
Lymphocytes
Inflammation

Keywords

  • Animal models of IBD
  • BALB/c
  • Microarray
  • SJL/J
  • Trinitrobenzene sulfonic acid (TNBS)

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Protective effects of dietary curcumin in mouse model of chemically induced colitis are strain dependent. / Larmonier, Claire B; Uno, Jennifer K.; Larmonier, Nicolas; Midura, Anna J.; Timmermann, Barbara; Ghishan, Fayez K; Kiela, Pawel R.

In: Inflammatory Bowel Diseases, Vol. 14, No. 6, 06.2008, p. 780-793.

Research output: Contribution to journalArticle

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abstract = "Background: Curcumin (diferulolylmethane) has been shown to have a protective role in mouse models of inflammatory bowel diseases (IBD) and to reduce the relapse rate in human ulcerative colitis (UC), thus making it a potentially viable supportive treatment option. Trinitrobenzene sulfonic acid (TNBS) colitis in NKT-deficient SJL/J mice has been described as Th1-mediated inflammation, whereas BALB/c mice are believed to exhibit a mixed Th1/Th2 response. Methods: We therefore investigated the effect of dietary curcumin in colitis induced in these 2 strains. Results: In the BALB/c mice, curcumin significantly increased survival, prevented weight loss, and normalized disease activity. In the SJL/J mice, curcumin demonstrated no protective effects. Genomewide microarray analysis of colonic gene expression was employed to define the differential effect of curcumin in these 2 strains. This analysis not only confirmed the disparate responses of the 2 strains to curcumin but also indicated different responses to TNBS. Curcumin inhibited proliferation of splenocytes from naive BALB/c mice but not SJL/J mice when nonspecifically stimulated in vitro with concanavalin A (ConA). Proliferation of CD4+ splenocytes was inhibited in both strains, albeit with about a 2-fold higher IC50 in SJL/J mice. Secretion of IL-4 and IL-5 by CD4+ lymphocytes of BALB/c mice but not SJL/J mice was significantly augmented by ConA and reduced to control levels by curcumin. Conclusions: The efficacy of dietary curcumin in TNBS colitis varies in BALB/c and SJL/J mouse strains. Although the exact mechanism underlying these differences is unclear, the results suggest that the therapeutic value of dietary curcumin may differ depending on the nature of immune dysregulation in IBD.",
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