Protein analysis of geographic isolates of shrimp white spot syndrome virus

Q. Wang, B. T. Poulos, Donald V Lightner

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Six geographic isolates of the white spot syndrome virus (WSSV) of penaeid shrimp, from China, India, Thailand, South Carolina, Texas, as well as from crayfish kept at the US National Zoo in Washington D. C, were compared by electron microscopy and sodium sulfate polyacrylamine gel electrophoresis (SDS-PAGE). Amino acid compositions of four of the major structural polypeptides of the South Carolina WSSV were analyzed, and three of the four polypeptides were partially sequenced from their NH2 termini. The morphologies of purified virions of the six geographic isolates of WSSV were indistinguishable by transmission electron microscopy. By SDS-PAGE, the protein profiles of the six isolates were very similar, but not identical. They all contained three major polypeptides with sizes of approximately 25, 23 and 19 kDa. A fourth major polypeptide at the 14.5 kDa position was observed in four of the geographic isolates. The WSSV isolated from crayfish presented a slightly different structural protein profile, particularly with regard to the protein in the 19 kDa range that appeared larger in size than those of the other isolates. The NH2 terminal amino acids of the 25, 23 and 14.5 kDa polypeptides of the South Carolina WSSV were sequenced as MDLSFTLSVVTA, MEFGNLTNLDVA, and VARGGKTKGRRG, respectively. No significant homologous sequence was found in the GenBank. These protein sequences have been submitted to the SWISS-PROT Protein Data Bank and assigned the accession numbers P82004, P82005 and P82006.

Original languageEnglish (US)
Pages (from-to)263-274
Number of pages12
JournalArchives of Virology
Volume145
Issue number2
StatePublished - 2000

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White spot syndrome virus 1
Peptides
Astacoidea
Proteins
Electrophoresis
Gels
Penaeidae
Amino Acids
Protein Databases
Nucleic Acid Databases
Thailand
Sequence Homology
Transmission Electron Microscopy
Virion
India
China
Electron Microscopy
Databases

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Protein analysis of geographic isolates of shrimp white spot syndrome virus. / Wang, Q.; Poulos, B. T.; Lightner, Donald V.

In: Archives of Virology, Vol. 145, No. 2, 2000, p. 263-274.

Research output: Contribution to journalArticle

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