Protein storage vacuoles of soybean aleurone cells accumulate a unique glycoprotein

Robert W. Yaklich, Eliot M Herman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The single cell-layer of aleurone cells exhibits structural evidence of high levels of Golgi apparatus mediated secretory activity [1]. We have used an antiserum directed against β-1,2-xyloglycan side chains of proteins as a probe of protein trafficking and Golgi activity. Although the aleurone contains many distinct xyloglycoproteins the primary xyloglycoprotein accumulated in the aleurone is a 42-kDa polypeptide. The 42-kDa xyloglycoprotein is accumulated in protein storage vacuoles within the aleurone cells. Comparison of anti-β-xyloglycoprotein immunoblots of the other embryonic tissues, cotyledon and axis, indicated that while numerous xyloglycoproteins appeared to be present in these tissues the 42-kDa xyloglycoprotein was absent. The identity of the protein storage vacuoles was confirmed by the presence of α-TIP (α-tonoplast integral protein) in the limiting membrane of the vacuoles. The aleurone also accumulates a variety of embryo-specific proteins including conglycinin, soybean agglutinin, and the oil body protein 24-kDa oleosin. The xyloglycoproteins appear to traffic through the Golgi apparatus. The anti-β-xyloglycoprotein serum labeled xyloglycoproteins in the medial-trans Golgi cisternae suggesting that the xylosylation of N-linked glycan was a medial-late processing event initiated in the medial Golgi cisternae. We interpret our results to indicate soybean aleurone cells accumulate a specific abundant xyloglycoprotein of unknown function in the cell's protein storage vacuole that appears to be unique to this single-cell layer of embryonic cells.

Original languageEnglish (US)
Pages (from-to)57-67
Number of pages11
JournalPlant Science
Volume107
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

aleurone cells
storage proteins
Vacuoles
Soybeans
vacuoles
glycoproteins
Glycoproteins
soybeans
Golgi apparatus
antiserum
Proteins
cells
oleosin
Golgi Apparatus
protein transport
proteins
lipid bodies
tonoplast
body protein
agglutinins

Keywords

  • Aleurone
  • Golgi apparatus
  • Immunocytochemistry
  • Protein storage vacuoles
  • Soybean
  • Xyloglycoprotein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Genetics
  • Biochemistry
  • Biotechnology

Cite this

Protein storage vacuoles of soybean aleurone cells accumulate a unique glycoprotein. / Yaklich, Robert W.; Herman, Eliot M.

In: Plant Science, Vol. 107, No. 1, 1995, p. 57-67.

Research output: Contribution to journalArticle

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