Protein storage vacuoles of soybean aleurone cells accumulate a unique glycoprotein

Robert W. Yaklich, Eliot M Herman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The single cell-layer of aleurone cells exhibits structural evidence of high levels of Golgi apparatus mediated secretory activity [1]. We have used an antiserum directed against β-1,2-xyloglycan side chains of proteins as a probe of protein trafficking and Golgi activity. Although the aleurone contains many distinct xyloglycoproteins the primary xyloglycoprotein accumulated in the aleurone is a 42-kDa polypeptide. The 42-kDa xyloglycoprotein is accumulated in protein storage vacuoles within the aleurone cells. Comparison of anti-β-xyloglycoprotein immunoblots of the other embryonic tissues, cotyledon and axis, indicated that while numerous xyloglycoproteins appeared to be present in these tissues the 42-kDa xyloglycoprotein was absent. The identity of the protein storage vacuoles was confirmed by the presence of α-TIP (α-tonoplast integral protein) in the limiting membrane of the vacuoles. The aleurone also accumulates a variety of embryo-specific proteins including conglycinin, soybean agglutinin, and the oil body protein 24-kDa oleosin. The xyloglycoproteins appear to traffic through the Golgi apparatus. The anti-β-xyloglycoprotein serum labeled xyloglycoproteins in the medial-trans Golgi cisternae suggesting that the xylosylation of N-linked glycan was a medial-late processing event initiated in the medial Golgi cisternae. We interpret our results to indicate soybean aleurone cells accumulate a specific abundant xyloglycoprotein of unknown function in the cell's protein storage vacuole that appears to be unique to this single-cell layer of embryonic cells.

Original languageEnglish (US)
Pages (from-to)57-67
Number of pages11
JournalPlant Science
Volume107
Issue number1
DOIs
Publication statusPublished - 1995
Externally publishedYes

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Keywords

  • Aleurone
  • Golgi apparatus
  • Immunocytochemistry
  • Protein storage vacuoles
  • Soybean
  • Xyloglycoprotein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Genetics
  • Biochemistry
  • Biotechnology

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