Proteogenomic mapping for structural annotation of prokaryote genomes

Nan Wang, Shane Burgess, Mark Lawrence, Susan Bridges

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Structural annotation of genomes is one of major goals of genomics research. Most popular tools for structural annotation of genomes are determined by computational pipelines. It is well-known that these computational methods have a number of shortcomings including false identifications and incorrect identification of gene boundaries. Proteomic data can used to confirm the identification of genes identified by computational methods and correct mistakes. A Proteogenomic mapping method has been developed, which uses peptides identified from mass spectrometry for structural annotation of genomes. Spectra are matched against both a protein database and the genome database translated in all six reading frames. Those peptides that match the genome but not the protein database potentially represent novel protein coding genes, annotation errors. These short experimentally derived peptides are used to discover potential novel protein coding genes called expressed Protein Sequence Tags (ePSTs) by aligning the peptides to the genomic DNA and extending the translation in the 3' and 5' direction. In the paper, an enhanced pipeline, has been designed and developed for discovering and evaluating of potential novel protein coding genes: 1) a distance-based outlier detection method for validating peptides identified from MS/MS, 2) a proteogenomic mapping for discovery of potential novel protein coding genes, 3) collection of evidence from a number of sources and automatically evaluate potential novel protein coding genes by using machine learning techniques, such as Neural Network, Support Vector Machine, Naïve Bayes etc.

Original languageEnglish (US)
Title of host publicationProceedings - 2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009
Pages103-106
Number of pages4
DOIs
StatePublished - Nov 26 2009
Externally publishedYes
Event2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009 - Shanghai, China
Duration: Aug 3 2009Aug 5 2009

Publication series

NameProceedings - 2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009

Other

Other2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009
CountryChina
CityShanghai
Period8/3/098/5/09

Keywords

  • Bayesian network
  • Expressed protein sequence tags
  • Naïve bayes
  • Neural network
  • Outlier detection
  • PST
  • Peptide validation
  • Potential genes
  • Proteogenomic mapping
  • Support vector machine
  • Target decoy strategy

ASJC Scopus subject areas

  • Software
  • Biomedical Engineering

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  • Cite this

    Wang, N., Burgess, S., Lawrence, M., & Bridges, S. (2009). Proteogenomic mapping for structural annotation of prokaryote genomes. In Proceedings - 2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009 (pp. 103-106). [5260732] (Proceedings - 2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009). https://doi.org/10.1109/IJCBS.2009.126