Proteome and membrane fatty acid analyses on oligotropha carboxidovorans OM5 grown under chemolithoautotrophic and heterotrophic conditions

Debarati Paul, Ranjit Kumar, Bindu Nanduri, Todd French, Ken Pendarvis, Ashli Brown, Mark L. Lawrence, Shane C. Burgess

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium able to utilize CO and H2 to derive energy for fixation of CO2. Thus, it is capable of growth using syngas, which is a mixture of varying amounts of CO and H2 generated by organic waste gasification. O. carboxidovorans is capable also of heterotrophic growth in standard bacteriologic media. Here we characterize how the O. carboxidovorans proteome adapts to different lifestyles of chemolithoautotrophy and heterotrophy. Fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown with acetate or with syngas showed that the bacterium changes membrane fatty acid composition. Quantitative shotgun proteomic analysis of O. carboxidovorans grown in the presence of acetate and syngas showed production of proteins encoded on the megaplasmid for assimilating CO and H2 as well as proteins encoded on the chromosome that might have contributed to fatty acid and acetate metabolism. We found that adaptation to chemolithoautotrophic growth involved adaptations in cell envelope, oxidative homeostasis, and metabolic pathways such as glyoxylate shunt and amino acid/cofactor biosynthetic enzymes.

Original languageEnglish (US)
Article numbere17111
JournalPloS one
Volume6
Issue number2
DOIs
StatePublished - 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint

Dive into the research topics of 'Proteome and membrane fatty acid analyses on oligotropha carboxidovorans OM5 grown under chemolithoautotrophic and heterotrophic conditions'. Together they form a unique fingerprint.

Cite this