Proteomic analysis of diaminochlorotriazine (DACT) adducts in three brain regions of Wistar Rats

Gregory P. Dooley, Amanda K. Ashley, Marie E. Legare, Robert J Handa, W. H. Hanneman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Atrazine (ATRA) is the most commonly applied herbicide in the United States and is detected frequently in drinking water at significant levels. Following oral exposure, metabolism of ATRA generates diaminochlorotriazine (DACT), an electrophilic molecule capable of forming covalent protein adducts. At high doses, both ATRA and DACT can disrupt the preovulatory luteinizing hormone (LH) surge in rats, thereby altering normal reproductive function. This research was designed to identify DACT protein adducts formed in three distinct brain regions of ATRA-exposed rats, including the preoptic area (POA), medial basal hypothalamus (MBH), and cortex (CTX). Proteins with DACT adducts were identified following 2-dimensional electrophoresis (2-DE), immunodetection, and MALDI-TOF mass spectrometry analysis. Western blots from exposed animals revealed over 30 DACT-modified spots that were absent in controls. Protein spots were matched to concurrently run 2-DE gels stained with Sypro Ruby, excised, and in-gel digested with trypsin.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalToxicology Letters
Volume199
Issue number1
DOIs
StatePublished - Nov 2010

Fingerprint

Atrazine
Proteomics
Wistar Rats
Rats
Brain
Electrophoresis
Proteins
Gels
Middle Hypothalamus
Preoptic Area
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Herbicides
Luteinizing Hormone
Metabolism
Drinking Water
Trypsin
Mass spectrometry
Mass Spectrometry
Animals
Western Blotting

Keywords

  • Atrazine
  • Brain
  • Chlorotriazines
  • DACT
  • Protein adducts

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

Proteomic analysis of diaminochlorotriazine (DACT) adducts in three brain regions of Wistar Rats. / Dooley, Gregory P.; Ashley, Amanda K.; Legare, Marie E.; Handa, Robert J; Hanneman, W. H.

In: Toxicology Letters, Vol. 199, No. 1, 11.2010, p. 17-21.

Research output: Contribution to journalArticle

Dooley, Gregory P. ; Ashley, Amanda K. ; Legare, Marie E. ; Handa, Robert J ; Hanneman, W. H. / Proteomic analysis of diaminochlorotriazine (DACT) adducts in three brain regions of Wistar Rats. In: Toxicology Letters. 2010 ; Vol. 199, No. 1. pp. 17-21.
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