Proteomic analysis of the reactive phenotype of astrocytes following endothelin-1 exposure

Gregory F. Egnaczyk, James D. Pomonis, Julie A. Schmidt, Scott D. Rogers, Christopher Peters, Joseph R. Ghilardi, Patrick W. Mantyh, John E. Maggio

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Reactive gliosis is an invariant feature of the pathology of central nervous system (CNS) injury and a major determinant of neuronal survival and regeneration. To begin to understand the alterations in astrocyte protein expression that drive glial changes that occur following injury, we used an established model system (endothelin-1 stimulation of hypertrophy) and proteomic analysis to define a discrete set of differentially expressed proteins and post-translational modifications that occur as the astrocytes change from a quiescent to a reactive state. This orchestrated set of changes included proteins involved in cytoskeletal reorganization (caldesmon, calponin, alpha B-crystallin, stathmin, collapsing response mediator protein-2), cell adhesion (vinculin, galectin-1), signal transduction (RACK-1) and astrocyte differentiation (glutamine synthetase). Using proteomic analysis to understand what drives astrocyte expression of these functionally divergent molecules may offer insight into the mechanisms by which astrocytes can exhibit both pro-regenerative and anti-regenerative activities following CNS injury.

Original languageEnglish (US)
Pages (from-to)689-698
Number of pages10
JournalProteomics
Volume3
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

Keywords

  • Astrocyte
  • Cytoskeleton
  • Endothelin
  • Hypertrophy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Egnaczyk, G. F., Pomonis, J. D., Schmidt, J. A., Rogers, S. D., Peters, C., Ghilardi, J. R., Mantyh, P. W., & Maggio, J. E. (2003). Proteomic analysis of the reactive phenotype of astrocytes following endothelin-1 exposure. Proteomics, 3(5), 689-698. https://doi.org/10.1002/pmic.200300407