Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages

Janet R. Donaldson, Bindu Nanduri, Joseph R. Pittman, Sumalee Givaruangsawat, Shane C Burgess, Mark L. Lawrence

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Listeria monocytogenes is able to survive and proliferate within macrophages. In the current study, the ability of three L. monocytogenes strains (serovar 1/2a strain EGDe, serovar 4b strain F2365, and serovar 4a strain HCC23) to proliferate in the murine macrophage cell line J774.1 was analyzed. We found that the avirulent strain HCC23 was able to initiate an infection but could not establish prolonged infection within the macrophages. By contrast, strains EGDe and F2365 proliferated within macrophages for at least 7. h. We further analyzed these strains by comparing their protein expression profiles at 0. h, 3. h, and 5. h post-infection using multidimensional protein identification technology coupled with electrospray ionization tandem mass spectrometry. Our results indicated that similar metabolic and cell wall associated proteins were expressed by all three strains at 3. h post-infection. However, increased expression of stress response and DNA repair proteins was associated with the ability to proliferate in macrophages at 5. h post-infection. By comparing the protein expression patterns of these three L. monocytogenes strains during intracellular growth in macrophages, we were able to detect biological differences that may determine the ability of L. monocytogenes to survive in macrophages.

Original languageEnglish (US)
Pages (from-to)1906-1917
Number of pages12
JournalJournal of Proteomics
Volume74
Issue number10
DOIs
StatePublished - Sep 6 2011
Externally publishedYes

Fingerprint

Listeria
Macrophages
Listeria monocytogenes
Proteomics
Infection
Proteins
Cells
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Tandem Mass Spectrometry
DNA Repair
Cell Wall
Mass spectrometry
Repair
Technology
Cell Line
DNA
Growth

Keywords

  • Intracellular
  • Listeria monocytogenes
  • Macrophages
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages. / Donaldson, Janet R.; Nanduri, Bindu; Pittman, Joseph R.; Givaruangsawat, Sumalee; Burgess, Shane C; Lawrence, Mark L.

In: Journal of Proteomics, Vol. 74, No. 10, 06.09.2011, p. 1906-1917.

Research output: Contribution to journalArticle

Donaldson, Janet R. ; Nanduri, Bindu ; Pittman, Joseph R. ; Givaruangsawat, Sumalee ; Burgess, Shane C ; Lawrence, Mark L. / Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages. In: Journal of Proteomics. 2011 ; Vol. 74, No. 10. pp. 1906-1917.
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