Protocadherins: A large family of cadherin-related molecules in central nervous system

K. Sano, H. Tanihara, R. L. Heimark, S. Obata, M. Davidson, T. St. John, S. Taketani, S. Suzuki

Research output: Contribution to journalArticlepeer-review

303 Scopus citations

Abstract

Using the polymerase chain reaction, we have isolated numerous rat and human cDNAs of which the deduced amino acid sequences are highly homologous to the sequences of the extracellular domain of cadherins. The entire putative coding sequences for two human proteins defined by two of these cDNAs have been determined. The overall structure of these molecules is very similar to that of classic cadherins, but they have some unique features. The extracellular domains are composed of six or seven subdomains that are very similar to those of cadherins, but have characteristic properties. The cytoplasmic domains, on the other hand, have no significant homology with those of classic cadherins. Since various cDNAs with almost identical features were obtained also from Xenopus, Drosophila and Caenorhabditis elegans, it appears that similar molecules are expressed in a variety of organisms. We have tentatively named these proteins protocadherins. They are highly expressed in brain and their expression appears to be developmentally regulated. The proteins expressed from the two full-length cDNAs in L cells were ~170 or 150 kDa in size, and were localized mainly at cell-cell contact sites. Moreover, the transfectants showed cell adhesion activity.

Original languageEnglish (US)
Pages (from-to)2249-2256
Number of pages8
JournalEMBO Journal
Volume12
Issue number6
DOIs
StatePublished - 1993

Keywords

  • Cadherins
  • Central nervous system
  • Extracellular domain
  • Protocadherins
  • Uvomorulia

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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