@article{2be38b3274244f53b1a57a6a60c0fb37,
title = "Pseudomonas syringae enhances herbivory by suppressing the reactive oxygen burst in Arabidopsis",
abstract = "Plant-herbivore interactions have evolved in the presence of plant-colonizing microbes. These microbes can have important third-party effects on herbivore ecology, as exemplified by drosophilid flies that evolved from ancestors feeding on plant-associated microbes. Leaf-mining flies in the genus Scaptomyza, which is nested within the paraphyletic genus Drosophila, show strong associations with bacteria in the genus Pseudomonas, including Pseudomonas syringae. Adult females are capable of vectoring these bacteria between plants and larvae show a preference for feeding on P. syringae-infected leaves. Here we show that Scaptomyza flava larvae can also vector P. syringae to and from feeding sites, and that they not only feed more, but also develop faster on plants previously infected with P. syringae. Our genetic and physiological data show that P. syringae enhances S. flava feeding on infected plants at least in part by suppressing anti-herbivore defenses mediated by reactive oxygen species.",
keywords = "Arabidopsis thaliana, Drosophila melanogaster, Herbivore, Plant defense, Pseudomonas syringae, Reactive oxygen species, Scaptomyza",
author = "Groen, {Simon C.} and Humphrey, {Parris T.} and Daniela Chevasco and Ausubel, {Frederick M.} and Pierce, {Naomi E.} and Whiteman, {Noah K.}",
note = "Funding Information: We thank Jennifer Bush and Andrew Gloss for providing Arabidopsis and B. vulgaris plants and adult S. flava , Hoon Pyon for assistance with experiments, and Paul Nabity for advice on conducting DAB staining. We are grateful to Andrew Gloss, Tim O{\textquoteright}Connor, Philippe Reymond and Sophie Zaaijer for valuable feedback on manuscript drafts and members of the Whiteman, Ausubel and Pierce laboratories for enlightening discussions. We acknowledge Matthew Velazquez for allowing the use of still images from his documentary about the Whiteman laboratory. NKW was supported by a Kirschstein National Research Service Award (NRSA) from the National Institutes of Health ( F32AI069732 to NKW), the National Geographic Society (Grant 9097-12 to NKW), and the University of Arizona (Faculty Seed Grant, Center for Insect Science Seed Grant, and laboratory set-up Grant to NKW). FMA and NKW were supported by Grants from the National Science Foundation (Grants MCB-0519898 and IOS 0929226 to FMA, and Grant DEB-1256758 to NKW), and FMA was supported by an R37 grant from the National Institutes of Health (Grant GM48707 to FMA). PTH and NKW were supported by the NSF (Grant DEB-1309493 to PTH and NKW). NKW, SCG, FMA and NEP were supported by a Grant from the Harvard University{\textquoteright}s Provost Fund for Interfaculty Collaboration, and a Grant from the Harvard University Science and Engineering Committee Seed Fund for Interdisciplinary Science . NKW and SCG were supported by the John Templeton Foundation (Grant ID #41855 to NKW). The funding sources had no involvement in the study design; the collection, analysis and interpretation of data; the writing of the report; or in the decision to submit the article for publication. Data accessibility: all raw data have been deposited in the Dryad data repository ( http://dx.doi.org/10.5061/dryad.7q37f ). Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",
year = "2016",
month = jan,
day = "1",
doi = "10.1016/j.jinsphys.2015.07.011",
language = "English (US)",
volume = "84",
pages = "90--102",
journal = "Journal of Insect Physiology",
issn = "0022-1910",
publisher = "Elsevier Limited",
}