Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps

J. R. Bonami, B. Trumper, J. Mari, M. Brehelin, Donald V Lightner

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physicochemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1.40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4.1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with M(r)s of 74K, 47K, 39K and 37.5K, respectively. From its characteristics, this virus could be a member of the Parvoviridae family.

Original languageEnglish (US)
Pages (from-to)2657-2664
Number of pages8
JournalJournal of General Virology
Volume71
Issue number11
StatePublished - 1990
Externally publishedYes

Fingerprint

Densovirinae
Penaeidae
Parvoviridae
Necrosis
Hematinics
Viruses
Virus Diseases
Agriculture
Transmission Electron Microscopy
Virion
Polyacrylamide Gel Electrophoresis
Genome
Peptides

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps. / Bonami, J. R.; Trumper, B.; Mari, J.; Brehelin, M.; Lightner, Donald V.

In: Journal of General Virology, Vol. 71, No. 11, 1990, p. 2657-2664.

Research output: Contribution to journalArticle

@article{66278e7c074e46a19bb9dc72d55030b9,
title = "Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps",
abstract = "Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physicochemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1.40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4.1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with M(r)s of 74K, 47K, 39K and 37.5K, respectively. From its characteristics, this virus could be a member of the Parvoviridae family.",
author = "Bonami, {J. R.} and B. Trumper and J. Mari and M. Brehelin and Lightner, {Donald V}",
year = "1990",
language = "English (US)",
volume = "71",
pages = "2657--2664",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "11",

}

TY - JOUR

T1 - Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps

AU - Bonami, J. R.

AU - Trumper, B.

AU - Mari, J.

AU - Brehelin, M.

AU - Lightner, Donald V

PY - 1990

Y1 - 1990

N2 - Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physicochemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1.40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4.1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with M(r)s of 74K, 47K, 39K and 37.5K, respectively. From its characteristics, this virus could be a member of the Parvoviridae family.

AB - Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physicochemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1.40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4.1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with M(r)s of 74K, 47K, 39K and 37.5K, respectively. From its characteristics, this virus could be a member of the Parvoviridae family.

UR - http://www.scopus.com/inward/record.url?scp=0025043678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025043678&partnerID=8YFLogxK

M3 - Article

C2 - 2254754

AN - SCOPUS:0025043678

VL - 71

SP - 2657

EP - 2664

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 11

ER -