Purine metabolism in pigs and humans and its implications for xenotransplantation

R. T. Smolenski, Zain I Khalpey, A. Yuen, H. Dziewit, E. M. Slominska, T. Borkowski, M. Zdunek, Z. Kochan, J. Karbowska, M. Lavitrano, M. H. Yacoub

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We compared concentrations of nucleotide substrates and activities of enzymes of nucleotide metabolism in pig and human blood, heart, and kidney. The most important difference was lower ecto-5′-nucleotidase (E5′N) activity in both pig hearts and kidney. Furthermore, higher hypoxanthine, inosine, adenine, and uracil, but lower undine and uric acid concentrations were observed in pig blood as compared to human. A twofold increase in UTP concentration has been observed in pig hearts following 4 h perfusion with human blood. Purine metabolism is an important target for genetic and pharmacological manipulation during xenotransplantations.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalNucleosides, Nucleotides and Nucleic Acids
Volume24
Issue number4
DOIs
StatePublished - 2005
Externally publishedYes

Fingerprint

Heterologous Transplantation
Metabolism
Blood
Swine
Nucleotides
Inosine
5'-Nucleotidase
Uridine Triphosphate
Hypoxanthine
Uracil
Adenine
Uric Acid
Kidney
Perfusion
Pharmacology
Substrates
Enzymes
purine

Keywords

  • ATP
  • Ecto-5′-Nucleotidase
  • Nucleotides
  • Purine Metabolites
  • UTP
  • Xenotransplantation

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Smolenski, R. T., Khalpey, Z. I., Yuen, A., Dziewit, H., Slominska, E. M., Borkowski, T., ... Yacoub, M. H. (2005). Purine metabolism in pigs and humans and its implications for xenotransplantation. Nucleosides, Nucleotides and Nucleic Acids, 24(4), 263-266. https://doi.org/10.1081/NCN-59701

Purine metabolism in pigs and humans and its implications for xenotransplantation. / Smolenski, R. T.; Khalpey, Zain I; Yuen, A.; Dziewit, H.; Slominska, E. M.; Borkowski, T.; Zdunek, M.; Kochan, Z.; Karbowska, J.; Lavitrano, M.; Yacoub, M. H.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 24, No. 4, 2005, p. 263-266.

Research output: Contribution to journalArticle

Smolenski, RT, Khalpey, ZI, Yuen, A, Dziewit, H, Slominska, EM, Borkowski, T, Zdunek, M, Kochan, Z, Karbowska, J, Lavitrano, M & Yacoub, MH 2005, 'Purine metabolism in pigs and humans and its implications for xenotransplantation', Nucleosides, Nucleotides and Nucleic Acids, vol. 24, no. 4, pp. 263-266. https://doi.org/10.1081/NCN-59701
Smolenski, R. T. ; Khalpey, Zain I ; Yuen, A. ; Dziewit, H. ; Slominska, E. M. ; Borkowski, T. ; Zdunek, M. ; Kochan, Z. ; Karbowska, J. ; Lavitrano, M. ; Yacoub, M. H. / Purine metabolism in pigs and humans and its implications for xenotransplantation. In: Nucleosides, Nucleotides and Nucleic Acids. 2005 ; Vol. 24, No. 4. pp. 263-266.
@article{ac954946d382426190bdd8cbdf9fdd5d,
title = "Purine metabolism in pigs and humans and its implications for xenotransplantation",
abstract = "We compared concentrations of nucleotide substrates and activities of enzymes of nucleotide metabolism in pig and human blood, heart, and kidney. The most important difference was lower ecto-5′-nucleotidase (E5′N) activity in both pig hearts and kidney. Furthermore, higher hypoxanthine, inosine, adenine, and uracil, but lower undine and uric acid concentrations were observed in pig blood as compared to human. A twofold increase in UTP concentration has been observed in pig hearts following 4 h perfusion with human blood. Purine metabolism is an important target for genetic and pharmacological manipulation during xenotransplantations.",
keywords = "ATP, Ecto-5′-Nucleotidase, Nucleotides, Purine Metabolites, UTP, Xenotransplantation",
author = "Smolenski, {R. T.} and Khalpey, {Zain I} and A. Yuen and H. Dziewit and Slominska, {E. M.} and T. Borkowski and M. Zdunek and Z. Kochan and J. Karbowska and M. Lavitrano and Yacoub, {M. H.}",
year = "2005",
doi = "10.1081/NCN-59701",
language = "English (US)",
volume = "24",
pages = "263--266",
journal = "Nucleosides and Nucleotides",
issn = "0732-8311",
publisher = "Taylor and Francis Ltd.",
number = "4",

}

TY - JOUR

T1 - Purine metabolism in pigs and humans and its implications for xenotransplantation

AU - Smolenski, R. T.

AU - Khalpey, Zain I

AU - Yuen, A.

AU - Dziewit, H.

AU - Slominska, E. M.

AU - Borkowski, T.

AU - Zdunek, M.

AU - Kochan, Z.

AU - Karbowska, J.

AU - Lavitrano, M.

AU - Yacoub, M. H.

PY - 2005

Y1 - 2005

N2 - We compared concentrations of nucleotide substrates and activities of enzymes of nucleotide metabolism in pig and human blood, heart, and kidney. The most important difference was lower ecto-5′-nucleotidase (E5′N) activity in both pig hearts and kidney. Furthermore, higher hypoxanthine, inosine, adenine, and uracil, but lower undine and uric acid concentrations were observed in pig blood as compared to human. A twofold increase in UTP concentration has been observed in pig hearts following 4 h perfusion with human blood. Purine metabolism is an important target for genetic and pharmacological manipulation during xenotransplantations.

AB - We compared concentrations of nucleotide substrates and activities of enzymes of nucleotide metabolism in pig and human blood, heart, and kidney. The most important difference was lower ecto-5′-nucleotidase (E5′N) activity in both pig hearts and kidney. Furthermore, higher hypoxanthine, inosine, adenine, and uracil, but lower undine and uric acid concentrations were observed in pig blood as compared to human. A twofold increase in UTP concentration has been observed in pig hearts following 4 h perfusion with human blood. Purine metabolism is an important target for genetic and pharmacological manipulation during xenotransplantations.

KW - ATP

KW - Ecto-5′-Nucleotidase

KW - Nucleotides

KW - Purine Metabolites

KW - UTP

KW - Xenotransplantation

UR - http://www.scopus.com/inward/record.url?scp=22044436408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22044436408&partnerID=8YFLogxK

U2 - 10.1081/NCN-59701

DO - 10.1081/NCN-59701

M3 - Article

C2 - 16021912

AN - SCOPUS:22044436408

VL - 24

SP - 263

EP - 266

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

SN - 0732-8311

IS - 4

ER -