pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

Andrew D. Johnson, Paul A. Krieg

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalGene
Volume147
Issue number2
DOIs
StatePublished - Sep 30 1994
Externally publishedYes

Keywords

  • EF-1α promoter
  • Plasmid
  • blastula
  • cat reporter
  • micro-injection
  • tadpole

ASJC Scopus subject areas

  • Genetics

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