pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

Andrew D. Johnson, Paul A Krieg

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalGene
Volume147
Issue number2
DOIs
StatePublished - Sep 30 1994
Externally publishedYes

Fingerprint

Peptide Elongation Factor 1
Xenopus
Plasmids
Embryonic Structures
Blastula
Nucleic Acid Regulatory Sequences
Xenopus laevis
Transcriptional Activation
Larva
Genome
Genes

Keywords

  • blastula
  • cat reporter
  • EF-1α promoter
  • micro-injection
  • Plasmid
  • tadpole

ASJC Scopus subject areas

  • Genetics

Cite this

pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos. / Johnson, Andrew D.; Krieg, Paul A.

In: Gene, Vol. 147, No. 2, 30.09.1994, p. 223-226.

Research output: Contribution to journalArticle

Johnson, Andrew D. ; Krieg, Paul A. / pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos. In: Gene. 1994 ; Vol. 147, No. 2. pp. 223-226.
@article{5d0852e1330c42e782ec4375fcf9fc08,
title = "pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos",
abstract = "We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.",
keywords = "blastula, cat reporter, EF-1α promoter, micro-injection, Plasmid, tadpole",
author = "Johnson, {Andrew D.} and Krieg, {Paul A}",
year = "1994",
month = "9",
day = "30",
doi = "10.1016/0378-1119(94)90070-1",
language = "English (US)",
volume = "147",
pages = "223--226",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

AU - Johnson, Andrew D.

AU - Krieg, Paul A

PY - 1994/9/30

Y1 - 1994/9/30

N2 - We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

AB - We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1α-encoding gene (EF-1α). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

KW - blastula

KW - cat reporter

KW - EF-1α promoter

KW - micro-injection

KW - Plasmid

KW - tadpole

UR - http://www.scopus.com/inward/record.url?scp=0028169246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028169246&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90070-1

DO - 10.1016/0378-1119(94)90070-1

M3 - Article

C2 - 7926804

AN - SCOPUS:0028169246

VL - 147

SP - 223

EP - 226

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -