Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice

Jianwei - Zhang, X. Li, G. Jiang, Y. Xu, Y. He

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

'Minghui 63' is a restorer line widely used in hybrid rice production in China for the last two decades. This line and its derived hybrids, including 'Shanyou 63', are susceptible to bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo). To improve the bacterial blight resistance of hybrid rice, two resistance genes Xa21 and Xa7, have been introgressed into 'Minghui 63' by marker-assisted selection and conventional backcrossing, respectively. The single resistance gene-introgressed lines, Minghui 63 (Xa21) and Minghui 63 (Xa7) had higher levels of resistance to bacterial blight than their derived hybrids, Shanyou 63 (Xa21) or Shanyou 63 (Xa7). Both Xa21 and Xa7 showed incomplete dominance in the heterozygous background of rice hybrids by infection with GX325 and KS-1-21. The improved restorer lines, with the homozygous genotypes, Xa21Xa21 or Xa7Xa7, were more resistant than their hybrids with the heterozygous genotypes Xa21xa21 or Xa7xa7. To further enhance the bacterial blight resistance of 'Minghui 63' and its hybrids, Xa21 and Xa7 were pyramided into the same background using molecular marker-aided selection. The restorer lines developed with the resistance genes Xa21 and Xa7, and their derived hybrids were evaluated for resistance after inoculation with 10 isolates of pathogens from China, Japan and the Philippines, and showed a higher level of resistance to BB than the restorer lines and derived hybrids having only one of the resistance genes. The pyramided double resistance lines and their derived hybrids have the same high level of resistance to BB. These results clearly indicate that pyramiding of dominant genes is a useful approach for improving BB resistance in hybrid rice.

Original languageEnglish (US)
Pages (from-to)600-605
Number of pages6
JournalPlant Breeding
Volume125
Issue number6
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Disease Resistance
blight
disease resistance
rice
restorer lines
Genes
China
Genotype
marker-assisted selection
Xanthomonas
Dominant Genes
Philippines
Inbreeding
Oryza
genes
incomplete dominance
Xanthomonas oryzae pv. oryzae
Japan
backcrossing
dominant genes

Keywords

  • Bacterial blight
  • Marker-assisted selection
  • Oryza sativa
  • Resistance gene pyramiding

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Biotechnology

Cite this

Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice. / Zhang, Jianwei -; Li, X.; Jiang, G.; Xu, Y.; He, Y.

In: Plant Breeding, Vol. 125, No. 6, 12.2006, p. 600-605.

Research output: Contribution to journalArticle

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AU - Zhang, Jianwei -

AU - Li, X.

AU - Jiang, G.

AU - Xu, Y.

AU - He, Y.

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AB - 'Minghui 63' is a restorer line widely used in hybrid rice production in China for the last two decades. This line and its derived hybrids, including 'Shanyou 63', are susceptible to bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo). To improve the bacterial blight resistance of hybrid rice, two resistance genes Xa21 and Xa7, have been introgressed into 'Minghui 63' by marker-assisted selection and conventional backcrossing, respectively. The single resistance gene-introgressed lines, Minghui 63 (Xa21) and Minghui 63 (Xa7) had higher levels of resistance to bacterial blight than their derived hybrids, Shanyou 63 (Xa21) or Shanyou 63 (Xa7). Both Xa21 and Xa7 showed incomplete dominance in the heterozygous background of rice hybrids by infection with GX325 and KS-1-21. The improved restorer lines, with the homozygous genotypes, Xa21Xa21 or Xa7Xa7, were more resistant than their hybrids with the heterozygous genotypes Xa21xa21 or Xa7xa7. To further enhance the bacterial blight resistance of 'Minghui 63' and its hybrids, Xa21 and Xa7 were pyramided into the same background using molecular marker-aided selection. The restorer lines developed with the resistance genes Xa21 and Xa7, and their derived hybrids were evaluated for resistance after inoculation with 10 isolates of pathogens from China, Japan and the Philippines, and showed a higher level of resistance to BB than the restorer lines and derived hybrids having only one of the resistance genes. The pyramided double resistance lines and their derived hybrids have the same high level of resistance to BB. These results clearly indicate that pyramiding of dominant genes is a useful approach for improving BB resistance in hybrid rice.

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KW - Marker-assisted selection

KW - Oryza sativa

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