Quadrupole magnetic sorting of porcine islets of langerhans

Rustin M. Shenkman, Jeffrey J. Chalmers, Bernhard J. Hering, Nicole Kirchhof, Klearchos K Papas

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Islet transplantation is emerging as a treatment option for selected patients with type 1 diabetes. Inconsistent isolation, purification, and recovery of large numbers of high-quality islets remain substantial impediments to progress in the field. Removing islets as soon as they are liberated from the pancreas during digestion and circumventing the need for density gradient purification is likely to result in substantially increased viable islet yields by minimizing exposure to proteolytic enzymes, reactive oxygen intermediates, and mechanical stress associated with centrifugation. This study capitalized on the hypervascularity of islets compared with acinar tissue to explore their preferential enrichment with magnetic beads to enable immediate separation in a magnetic field utilizing a quadrupole magnetic sorting. The results demonstrate that (1) preferential enrichment of porcine islets is achievable, but homogeneous bead distribution within the pancreas is difficult to achieve with current protocols; (2) greater than 70% of islets in the dissociated pancreatic tissue were recovered by quadrupole magnetic sorting, but their purity was low; and (3) infused islets purified by density gradients and subsequently passed through quadrupole magnetic sorting had similar potency as uninfused islets. These results demonstrate proof of concept and define the steps for implementation of this technology in pig and human islet isolation.

Original languageEnglish (US)
Pages (from-to)147-156
Number of pages10
JournalTissue Engineering - Part C: Methods
Volume15
Issue number2
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

Fingerprint

Islets of Langerhans
Sorting
Pancreas
Swine
Islets of Langerhans Transplantation
Mechanical Stress
Purification
Magnetic Fields
Type 1 Diabetes Mellitus
Centrifugation
Tissue
Digestion
Peptide Hydrolases
Medical problems
Oxygen
Technology
Magnetic fields
Recovery
Therapeutics

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Quadrupole magnetic sorting of porcine islets of langerhans. / Shenkman, Rustin M.; Chalmers, Jeffrey J.; Hering, Bernhard J.; Kirchhof, Nicole; Papas, Klearchos K.

In: Tissue Engineering - Part C: Methods, Vol. 15, No. 2, 01.06.2009, p. 147-156.

Research output: Contribution to journalArticle

Shenkman, Rustin M. ; Chalmers, Jeffrey J. ; Hering, Bernhard J. ; Kirchhof, Nicole ; Papas, Klearchos K. / Quadrupole magnetic sorting of porcine islets of langerhans. In: Tissue Engineering - Part C: Methods. 2009 ; Vol. 15, No. 2. pp. 147-156.
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