Qualitative thrombelastographic detection of tissue factor in human plasma

Vance G. Nielsen, Paul Audu, Lana Cankovic, Ralph T. Lyerly, Brad L. Steenwyk, Valerie Armstead, Garry Powell

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

BACKGROUND: Tissue factor (TF) is the principal in vivo initiator of coagulation, with normal circulating TF concentrations reported to be approximately 23-158 pg/mL. However, patients with atherosclerosis or cancer have been reported to have TF concentrations ranging between 800 and 9000 pg/mL. Of interest, thrombelastographic (TEG®)-based measures of clot initiation and propagation have demonstrated hypercoagulability in such patients at risk for thromboembolic events. Thus, our goal in the present investigation was to establish a concentration-response relationship of the effect of TF on TEG® variables, and determine specificity of TF-mediated events with a monoclonal TF antibody. METHODS: Thrombelastography was performed on normal human plasma exposed to 0, 500, 1000, or 2000 pg/mL TF. Additional experiments with plasma exposed to 0 or 750 pg/mL TF in the presence or absence of a monoclonal TF antibody (1:360 dilution, 10 min incubation) were also performed. Clot initiation time (R) and the speed of clot propagation (MRTG, maximum rate of thrombus generation) were determined. RESULTS: The addition of TF to normal plasma resulted in a significant, concentration-dependent decrease in R and increase MRTG values. The addition of TF antibody to samples with TF significantly increased R and decreased MRTG values compared to samples with TF addition. CONCLUSIONS: In conclusion, changes in TEG® variables in conjunction with use of a TF antibody can detect pathological concentrations of TF in human plasma in vitro. Further investigation is warranted to determine if TEG®-based monitoring could assist in the detection and prevention of TF-initiated thromboembolic events.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalAnesthesia and analgesia
Volume104
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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    Nielsen, V. G., Audu, P., Cankovic, L., Lyerly, R. T., Steenwyk, B. L., Armstead, V., & Powell, G. (2007). Qualitative thrombelastographic detection of tissue factor in human plasma. Anesthesia and analgesia, 104(1), 59-64. https://doi.org/10.1213/01.ane.0000248223.05152.a1