Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia

Melissa Herbst-Kralovetz, Alison J. Quayle, Mercedes Ficarra, Sheila Greene, William A. Rose, Ralph Chesson, Rae Ann Spagnuolo, Richard B. Pyles

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Problem: To better understand innate immune responses to sexually-transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll-like receptor (TLR) expression from a population of women is needed. Methods of study: TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration. Results: TLR1-3 and 5-9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7-9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels. Conclusion: TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.

Original languageEnglish (US)
Pages (from-to)212-224
Number of pages13
JournalAmerican Journal of Reproductive Immunology
Volume59
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Toll-Like Receptors
Epithelium
Epithelial Cells
Cytokines
Sexually Transmitted Diseases
Innate Immunity
Population
Mucous Membrane
Tissue Donors
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia. / Herbst-Kralovetz, Melissa; Quayle, Alison J.; Ficarra, Mercedes; Greene, Sheila; Rose, William A.; Chesson, Ralph; Spagnuolo, Rae Ann; Pyles, Richard B.

In: American Journal of Reproductive Immunology, Vol. 59, No. 3, 03.2008, p. 212-224.

Research output: Contribution to journalArticle

Herbst-Kralovetz, Melissa ; Quayle, Alison J. ; Ficarra, Mercedes ; Greene, Sheila ; Rose, William A. ; Chesson, Ralph ; Spagnuolo, Rae Ann ; Pyles, Richard B. / Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia. In: American Journal of Reproductive Immunology. 2008 ; Vol. 59, No. 3. pp. 212-224.
@article{888fb2c6854c435e85a96c1a6cbf1590,
title = "Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia",
abstract = "Problem: To better understand innate immune responses to sexually-transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll-like receptor (TLR) expression from a population of women is needed. Methods of study: TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration. Results: TLR1-3 and 5-9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7-9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels. Conclusion: TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.",
author = "Melissa Herbst-Kralovetz and Quayle, {Alison J.} and Mercedes Ficarra and Sheila Greene and Rose, {William A.} and Ralph Chesson and Spagnuolo, {Rae Ann} and Pyles, {Richard B.}",
year = "2008",
month = "3",
doi = "10.1111/j.1600-0897.2007.00566.x",
language = "English (US)",
volume = "59",
pages = "212--224",
journal = "American Journal of Reproductive Immunology and Microbiology",
issn = "8755-8920",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia

AU - Herbst-Kralovetz, Melissa

AU - Quayle, Alison J.

AU - Ficarra, Mercedes

AU - Greene, Sheila

AU - Rose, William A.

AU - Chesson, Ralph

AU - Spagnuolo, Rae Ann

AU - Pyles, Richard B.

PY - 2008/3

Y1 - 2008/3

N2 - Problem: To better understand innate immune responses to sexually-transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll-like receptor (TLR) expression from a population of women is needed. Methods of study: TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration. Results: TLR1-3 and 5-9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7-9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels. Conclusion: TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.

AB - Problem: To better understand innate immune responses to sexually-transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll-like receptor (TLR) expression from a population of women is needed. Methods of study: TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration. Results: TLR1-3 and 5-9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7-9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels. Conclusion: TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.

UR - http://www.scopus.com/inward/record.url?scp=39149137535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39149137535&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0897.2007.00566.x

DO - 10.1111/j.1600-0897.2007.00566.x

M3 - Article

C2 - 18201283

AN - SCOPUS:39149137535

VL - 59

SP - 212

EP - 224

JO - American Journal of Reproductive Immunology and Microbiology

JF - American Journal of Reproductive Immunology and Microbiology

SN - 8755-8920

IS - 3

ER -