Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Patharapol Piamsomboon, Seong Kyoon Choi, Bambang Hanggono, Yani Lestari Nuraini, Fatma Wati, Kathy F.J. Tang, Song Park, Dongmi Kwak, Man Hee Rhee, Jee Eun Han, Ji Hyung Kim

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

Original languageEnglish (US)
Article number233
JournalPathogens
Volume8
Issue number4
DOIs
StatePublished - Dec 2019

Keywords

  • Enterocytozoon hepatopenaei
  • Microsporidian
  • QPCR
  • Shrimp parasite

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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