Quantifying re-association of a facultative endohyphal bacterium with a filamentous fungus

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We present here a method to quantify reassociation between facultative endohyphal bacteria and filamentous fungal hosts. Our method takes advantage of the capabilities of fungal cell walls to selectively protect internal bacteria from gentamicin treatment, an assay adapted from studies of internalized bacterial pathogens in cell culture. We report the efficacy of gentamicin to kill planktonic bacteria treated during fungal coculture, and also describe and characterize a sampling scheme to recover and quantify culturable bacteria from the growing edge of fungal mycelium in vitro. This assay enables qualitative and quantitative tests of reassociation capabilities for facultative endohyphal bacteria with host fungi and provides a means to investigate the genetic basis for these associations in a repeatable way.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-11
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1848
ISSN (Print)1064-3745

Keywords

  • Ascomycete
  • Endofungal bacteria
  • Endohyphal bacteria
  • Reassociation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Baltrus, D. A., Spraker, J., & Arnold, A. E. (2018). Quantifying re-association of a facultative endohyphal bacterium with a filamentous fungus. In Methods in Molecular Biology (pp. 1-11). (Methods in Molecular Biology; Vol. 1848). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8724-5_1