Quantitation of hepatitis A virus and enterovirus levels in the lagoon canals and Lido beach of Venice, Italy, using real-time RT-PCR

Michael A. Rose, Arun K. Dhar, Hilary A. Brooks, Fulvio Zecchini, Richard M. Gersberg

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

In order to assess the microbial water quality of the lagoon canals of Venice, Italy and nearby beach on Lido island, a study was conducted using real-time RT-PCR to enumerate levels of hepatitis A virus (HAV) and enteroviruses in these marine waters over a 3-year period from 2003 to 2005. A total of 17 sites (9 lagoon canal and 8 beach sites) were assayed. For the canals of the Venice Lagoon, 78% were positive for both HAV and enteroviruses, with levels ranging from 75 to 730 and 3 to 1614 genome copies/L, respectively. At Lido beach, HAV was never detected, but enteroviruses were detected in all Lido beach samples at levels ranging from 2 to 71 genome copies/L. There was a statistically significant correlation between thermotolerant coliform densities and HAV levels (p=0.0002), but the relationship between thermotolerant coliform densities and enterovirus levels was not significant (p>0.05). Despite the fact that enteroviruses were detected at low levels in the surfzone at Lido beach, the risk for enteroviral infection (calculated using the beta-Poisson model) for recreational exposure from swimming, was in the range of 1.9×10-3-6.1×10-2, yielding a disease risk at or below the level (5% for gastroenteritis) deemed acceptable by European Guide standards.

Original languageEnglish (US)
Pages (from-to)2387-2396
Number of pages10
JournalWater research
Volume40
Issue number12
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • Enteroviruses
  • Hepatitis a virus
  • Real-time RT-PCR
  • Risk assessment
  • Venice Lagoon

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Fingerprint Dive into the research topics of 'Quantitation of hepatitis A virus and enterovirus levels in the lagoon canals and Lido beach of Venice, Italy, using real-time RT-PCR'. Together they form a unique fingerprint.

  • Cite this