Quantitation of xylose from plasma and urine by capillary column gas chromatography

Stephen L. Johnson, Michael Mayersohn

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A specific and sensitive assay for quantitation of xylose from plasma and urine has been developed. Following a clean-up procedure, plasma (0.1 ml) or urine (0.2 ml) samples are concentrated and undergo two sequential derivatization steps. A methyloxime derivative is formed initially, followed by trimethylsilylation of all hydroxyl groups. The derivatized samples are quantitated by capillary column gas chromatography using flame ionization detection. Xylose and the internal standard (2-deoxy-d-ribose) have retention times of 6.5 and 5.2 min, respectively. Other monosaccharides (e.g. ribose, arabinose) do not interfere with the assay. Standard curves are linear and reproducible over a concentration range of 10-200 mg/l for plasma and 100-2000 mg/l for urine. The within-day and day-to-day percentage coefficients of variation were less than 5 and 9%, respectively, for plasma and urine.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalClinica Chimica Acta
Volume137
Issue number1
DOIs
StatePublished - Feb 14 1984

Fingerprint

Column chromatography
Xylose
Gas chromatography
Gas Chromatography
Urine
Plasmas
Ribose
Assays
Flame Ionization
Arabinose
Monosaccharides
Hydroxyl Radical
Ionization
Derivatives

Keywords

  • Aldose
  • Methyloxime derivate
  • Monosaccharide
  • Pentose
  • Xylose

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Quantitation of xylose from plasma and urine by capillary column gas chromatography. / Johnson, Stephen L.; Mayersohn, Michael.

In: Clinica Chimica Acta, Vol. 137, No. 1, 14.02.1984, p. 13-20.

Research output: Contribution to journalArticle

@article{7b228da05380432a91fc5fa938036bb5,
title = "Quantitation of xylose from plasma and urine by capillary column gas chromatography",
abstract = "A specific and sensitive assay for quantitation of xylose from plasma and urine has been developed. Following a clean-up procedure, plasma (0.1 ml) or urine (0.2 ml) samples are concentrated and undergo two sequential derivatization steps. A methyloxime derivative is formed initially, followed by trimethylsilylation of all hydroxyl groups. The derivatized samples are quantitated by capillary column gas chromatography using flame ionization detection. Xylose and the internal standard (2-deoxy-d-ribose) have retention times of 6.5 and 5.2 min, respectively. Other monosaccharides (e.g. ribose, arabinose) do not interfere with the assay. Standard curves are linear and reproducible over a concentration range of 10-200 mg/l for plasma and 100-2000 mg/l for urine. The within-day and day-to-day percentage coefficients of variation were less than 5 and 9{\%}, respectively, for plasma and urine.",
keywords = "Aldose, Methyloxime derivate, Monosaccharide, Pentose, Xylose",
author = "Johnson, {Stephen L.} and Michael Mayersohn",
year = "1984",
month = "2",
day = "14",
doi = "10.1016/0009-8981(84)90307-3",
language = "English (US)",
volume = "137",
pages = "13--20",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Quantitation of xylose from plasma and urine by capillary column gas chromatography

AU - Johnson, Stephen L.

AU - Mayersohn, Michael

PY - 1984/2/14

Y1 - 1984/2/14

N2 - A specific and sensitive assay for quantitation of xylose from plasma and urine has been developed. Following a clean-up procedure, plasma (0.1 ml) or urine (0.2 ml) samples are concentrated and undergo two sequential derivatization steps. A methyloxime derivative is formed initially, followed by trimethylsilylation of all hydroxyl groups. The derivatized samples are quantitated by capillary column gas chromatography using flame ionization detection. Xylose and the internal standard (2-deoxy-d-ribose) have retention times of 6.5 and 5.2 min, respectively. Other monosaccharides (e.g. ribose, arabinose) do not interfere with the assay. Standard curves are linear and reproducible over a concentration range of 10-200 mg/l for plasma and 100-2000 mg/l for urine. The within-day and day-to-day percentage coefficients of variation were less than 5 and 9%, respectively, for plasma and urine.

AB - A specific and sensitive assay for quantitation of xylose from plasma and urine has been developed. Following a clean-up procedure, plasma (0.1 ml) or urine (0.2 ml) samples are concentrated and undergo two sequential derivatization steps. A methyloxime derivative is formed initially, followed by trimethylsilylation of all hydroxyl groups. The derivatized samples are quantitated by capillary column gas chromatography using flame ionization detection. Xylose and the internal standard (2-deoxy-d-ribose) have retention times of 6.5 and 5.2 min, respectively. Other monosaccharides (e.g. ribose, arabinose) do not interfere with the assay. Standard curves are linear and reproducible over a concentration range of 10-200 mg/l for plasma and 100-2000 mg/l for urine. The within-day and day-to-day percentage coefficients of variation were less than 5 and 9%, respectively, for plasma and urine.

KW - Aldose

KW - Methyloxime derivate

KW - Monosaccharide

KW - Pentose

KW - Xylose

UR - http://www.scopus.com/inward/record.url?scp=0021325591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021325591&partnerID=8YFLogxK

U2 - 10.1016/0009-8981(84)90307-3

DO - 10.1016/0009-8981(84)90307-3

M3 - Article

C2 - 6421511

AN - SCOPUS:0021325591

VL - 137

SP - 13

EP - 20

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1

ER -