Abstract
A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.
Original language | English (US) |
---|---|
Pages (from-to) | 2-6 |
Number of pages | 5 |
Journal | Journal of Analytical Toxicology |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - 1984 |
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry
- Toxicology
- Health, Toxicology and Mutagenesis
- Chemical Health and Safety