Abstract
A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2-6 |
| Number of pages | 5 |
| Journal | Journal of Analytical Toxicology |
| Volume | 8 |
| Issue number | 1 |
| State | Published - 1984 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Health, Toxicology and Mutagenesis
- Toxicology
Cite this
Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography. / Woodworth, J. R.; Mayersohn, Michael; Owens, S. M.
In: Journal of Analytical Toxicology, Vol. 8, No. 1, 1984, p. 2-6.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography
AU - Woodworth, J. R.
AU - Mayersohn, Michael
AU - Owens, S. M.
PY - 1984
Y1 - 1984
N2 - A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.
AB - A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.
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M3 - Article
C2 - 6708471
AN - SCOPUS:0021321708
VL - 8
SP - 2
EP - 6
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
SN - 0146-4760
IS - 1
ER -