Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography

J. R. Woodworth, M. Mayersohn, S. M. Owens

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.

Original languageEnglish (US)
Pages (from-to)2-6
Number of pages5
JournalJournal of Analytical Toxicology
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Fingerprint Dive into the research topics of 'Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography'. Together they form a unique fingerprint.

  • Cite this