Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography

J. R. Woodworth, Michael Mayersohn, S. M. Owens

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A sensitive capillary gas chromatography (GC) procedure was developed for the analysis of phencyclidine (PCP), two of its monohydroxy-metabolites, and a recently identified pentanoic acid metabolite. Two separate, but sequential, integrated extraction techniques were necessary to isolate all of the compounds from a 1.0-mL biological sample. Two GC techniques were necessary to analyze all the compounds; both methods used a capillary column and a nitrogen phosphorus detector (NPD). The first procedure permitted isolation and quantitation of PCP and two monohydroxy-metabolites. A second extraction of the biological samples permitted measurement of the pentanoic acid metabolite. The analytical methods illustrate good reproducibility based on within-day and day-to-day variation. Serum and urine samples were analyzed from a dog administered PCP to illustrate the utility of this procedure.

Original languageEnglish (US)
Pages (from-to)2-6
Number of pages5
JournalJournal of Analytical Toxicology
Volume8
Issue number1
StatePublished - 1984

Fingerprint

Phencyclidine
Column chromatography
Metabolites
Gas chromatography
Gas Chromatography
quantitative analysis
metabolite
gas chromatography
PCP
Pentanoic Acids
Chemical analysis
Acids
acid
urine
Phosphorus
serum
analytical method
Nitrogen
phosphorus
Detectors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Quantitative analysis of phencyclidine and metabolites by capillary column gas chromatography. / Woodworth, J. R.; Mayersohn, Michael; Owens, S. M.

In: Journal of Analytical Toxicology, Vol. 8, No. 1, 1984, p. 2-6.

Research output: Contribution to journalArticle

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