Quantitative analysis of Streptococcus pneumoniae TIGR4 response to in vitro iron restriction by 2-D LC ESI MS/MS

Bindu Nanduri, Pratik Shah, Mahalingam Ramkumar, Edward B. Allen, Edwin Swiatlo, Shane C. Burgess, Mark L. Lawrence

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Understanding the growth of bacterial pathogens in a micronutrient restricted host environment can identify potential virulence proteins that help overcome this nutritional barrier to productive infection. In this study, we investigated the pneumococcal protein expression response to iron limitation using an in vitro model. We identified S. pneumoniae TIGR4 proteins by 2-D LC ESI MS/MS and determined significant changes in protein expression in response to iron restriction using computer-intensive random resampling methods. Differential protein expression was studied in the context of a S. pneumoniae TIGR4 protein interaction network using Pathway Studio. Our analysis showed that pneumococcal iron restriction response was marked by increased expression of known virulence factors like PsaA. It involved changes in the expression of stress response, and phase variation and biofilm formation proteins. The net effect of changes in all these biological processes could increase the virulence of S. pneumoniae TIGR4 during in vivo infection.

Original languageEnglish (US)
Pages (from-to)2104-2114
Number of pages11
Issue number10
StatePublished - May 2008
Externally publishedYes


  • Interaction networks
  • Iron restriction
  • Monte Carlo
  • Pathogenesis
  • Random resampling with replacement

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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