Quantitative approaches for assessing chromosome loss in Saccharomyces cerevisiae: general methods for analyzing downturns in dose response

Walter W. Piegorsch, Friedrich K. Zimmermann, Seymour Fogel, Stephen G. Whittaker, Michael A. Resnick

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Statistical methods are considered for analysis of data arising from a mitotic chromosome loss assay in Saccharomyces cerevisiae strain D61.M. The methods make use of reproducibility trial data from the assay (presented herein) and previous data, which suggest a unimodal, 'umbrella-patterned' dose response. Computer simulations are employed to illustrate the operating characteristics of the umbrella response methods. These methods are generally applicable to any toxicity assay that exhibits a downturn in dose response. Experimental design considerations are also discussed. These include applications of 2-stage sampling rules to first gauge the dose window of peak response, then test if the response deviates significantly from untreated levels.

Original languageEnglish (US)
Pages (from-to)11-29
Number of pages19
JournalMutation Research/Genetic Toxicology
Volume224
Issue number1
DOIs
StatePublished - Sep 1989
Externally publishedYes

Keywords

  • Aneuploidy
  • Chromosome loss
  • Control reproducibility trials
  • Non-parametric statistical tests
  • Umbrella alternatives

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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