Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles

M. T. Stamm, A. S. Trickey-Glassman, L. Jiang, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Receptor-ligand binding has been one of the more popular approaches to specifically targeting tumor cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM-expressing BT-20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalized particles bound to target cells were experimentally investigated. The number of bound particles was found to increase with particle concentration, but not necessarily with incubation time. While particle desorption and cellular loss of binding affinity in time seem to be negligible, cell-particle-cell interaction was identified as the limiting mechanism for the number of particles bound to target cells. The current findings suggest that separation of a bound particle from a cell may be detrimental to cellular binding affinity.

Original languageEnglish (US)
Title of host publication8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages1108-1111
Number of pages4
DOIs
StatePublished - 2013
Event8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China
Duration: Apr 7 2013Apr 10 2013

Other

Other8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
CountryChina
CitySuzhou
Period4/7/134/10/13

Fingerprint

Neoplasm Antibodies
Tumors
Cells
Antibodies
Silicon Dioxide
Desorption
Ligands
Cell Communication
Breast Neoplasms
Neoplasms

Keywords

  • functionalized particles
  • microfluidics
  • particle-cell binding
  • receptor-ligand interaction
  • specific targeting

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biotechnology

Cite this

Stamm, M. T., Trickey-Glassman, A. S., Jiang, L., & Zohar, Y. (2013). Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 (pp. 1108-1111). [6559916] https://doi.org/10.1109/NEMS.2013.6559916

Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles. / Stamm, M. T.; Trickey-Glassman, A. S.; Jiang, L.; Zohar, Yitshak.

8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. p. 1108-1111 6559916.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stamm, MT, Trickey-Glassman, AS, Jiang, L & Zohar, Y 2013, Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles. in 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013., 6559916, pp. 1108-1111, 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013, Suzhou, China, 4/7/13. https://doi.org/10.1109/NEMS.2013.6559916
Stamm MT, Trickey-Glassman AS, Jiang L, Zohar Y. Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. p. 1108-1111. 6559916 https://doi.org/10.1109/NEMS.2013.6559916
Stamm, M. T. ; Trickey-Glassman, A. S. ; Jiang, L. ; Zohar, Yitshak. / Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. pp. 1108-1111
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