Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles

M. T. Stamm, A. S. Trickey-Glassman, L. Jiang, Y. Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Receptor-ligand binding has been one of the more popular approaches to specifically targeting tumor cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM-expressing BT-20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalized particles bound to target cells were experimentally investigated. The number of bound particles was found to increase with particle concentration, but not necessarily with incubation time. While particle desorption and cellular loss of binding affinity in time seem to be negligible, cell-particle-cell interaction was identified as the limiting mechanism for the number of particles bound to target cells. The current findings suggest that separation of a bound particle from a cell may be detrimental to cellular binding affinity.

Original languageEnglish (US)
Title of host publication8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages1108-1111
Number of pages4
DOIs
StatePublished - Sep 2 2013
Event8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China
Duration: Apr 7 2013Apr 10 2013

Publication series

Name8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013

Other

Other8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
CountryChina
CitySuzhou
Period4/7/134/10/13

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Keywords

  • functionalized particles
  • microfluidics
  • particle-cell binding
  • receptor-ligand interaction
  • specific targeting

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biotechnology

Cite this

Stamm, M. T., Trickey-Glassman, A. S., Jiang, L., & Zohar, Y. (2013). Quantitative characterization of specific targeting of tumor cells by antibody-functionalized particles. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 (pp. 1108-1111). [6559916] (8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013). https://doi.org/10.1109/NEMS.2013.6559916