Quantitative chemical analysis of single cells.

Michael L Heien, Andrew G. Ewing

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Exocytosis, the fusion of intracellular vesicles with the membrane and subsequent release of vesicular contents, is important in intercellular communication. The release event is a rapid process (milliseconds), hence detection of released chemicals requires a detection scheme that is both sensitive and has rapid temporal dynamics. Electrochemistry at carbon-fiber microelectrodes allows time-resolved exocytosis of electroactive catecholamines to be observed at very low levels. When coupled with constant-potential amperometry, the number of molecules released and the kinetics of quantal release can be determined. The rapid response time (milliseconds) of microelectrodes makes them well suited for monitoring the dynamic process of exocytosis.

Original languageEnglish (US)
Pages (from-to)153-162
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume544
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Single-Cell Analysis
Exocytosis
Microelectrodes
Electrochemistry
Reaction Time
Catecholamines
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Quantitative chemical analysis of single cells. / Heien, Michael L; Ewing, Andrew G.

In: Methods in molecular biology (Clifton, N.J.), Vol. 544, 2009, p. 153-162.

Research output: Contribution to journalArticle

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